The upstream regulatory region of the gene for the human homologue of the adhesion molecule TAG-1 contains elements driving neural specific expression in vivo

• Published in: Brain research. Molecular brain research. 2003-10, Vol.118 (1), p.91-101
• Main Authors: Denaxa, Myrto, Pavlou, Ourania, Tsiotra, Panayoula, Papadopoulos, Georgios C., Liapaki, Katerina, Theodorakis, Kostas, Papadaki, Chara, Karagogeos, Domna, Papamatheakis, Joseph
• Format: Article
• Language: English
• Subjects: Aging - genetics; Animals; Animals, Newborn; Base Sequence - genetics; Biological and medical sciences; Cell Adhesion - genetics; Cell adhesion molecules; Cell Adhesion Molecules, Neuronal - genetics; Cell Differentiation - genetics; Contactin 2; Fetus; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental - genetics; Genes, Reporter - genetics; Genes. Genome; Humans; Immunoglobulin superfamily; Lac Operon - genetics; Membrane Glycoproteins - genetics; Mice; Mice, Transgenic; Molecular and cellular biology; Molecular genetics; Nervous System - embryology; Nervous System - growth & development; Nervous System - metabolism; Neurons - cytology; Neurons - metabolism; Regulatory sequences; Regulatory Sequences, Nucleic Acid - genetics; RNA, Messenger - metabolism; TAG-1/axonin-1; Transgenes - genetics; Transgenesis
• ISSN: 0169-328X; 1872-6941
• DOI: 10.1016/j.molbrainres.2003.07.004

Cell adhesion molecules (CAMs) of the immunoglobulin superfamily (IgSF) exhibit restricted spatial and temporal expression profiles requiring a tight regulatory program during development. The rodent glycoprotein TAG-1 and its orthologs TAX-1 in the human and axonin-1 in chick are cell adhesion molecules belonging to the contactin/F3 subgroup of the IgSF. TAG-1 is expressed in restricted subsets of central and peripheral neurons, not only during development but also in adulthood, and is implicated in neurite outgrowth, axon guidance and fasciculation, as well as neuronal migration. In an attempt to identify the regulatory elements that guide the neuronal expression of TAG-1, we have isolated genomic clones containing 4 kb of the TAX-1 upstream sequence and used them to drive the expression of the LacZ reporter gene in transgenic mice. We demonstrate that this sequence includes elements not only sufficient to restrict expression to the nervous system, but also to recapitulate to a great extent the endogenous pattern of the TAG-1 expression in the developing CNS.