Identification of type 4 fimbriae in Actinobacillus pleuropneumoniae

Abstract Type 4 fimbriae have been identified on the cell surface of Actinobacillus pleuropneumoniae by electron microscopy and N-terminal sequencing analysis. A. pleuropneumoniae type 4 fimbrial subunit protein, purified from cell cultures and from outer membrane preparations, reacted with polyclon...

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Bibliographic Details
Published in:FEMS microbiology letters 2000-08, Vol.189 (1), p.15-18
Main Authors: Zhang, Yamei, Tennent, Jan M, Ingham, Aaron, Beddome, Gary, Prideaux, Christopher, Michalski, Wojtek P
Format: Article
Language:English
Subjects:
Actinobacillus pleuropneumoniae
Actinobacillus pleuropneumoniae - metabolism
Actinobacillus pleuropneumoniae - ultrastructure
Amino acids
Animals
Antibodies
Bacterial Outer Membrane Proteins - chemistry
Bacterial Outer Membrane Proteins - isolation & purification
Bacteriology
Biological and medical sciences
Blotting, Western
Cell surface
Electron microscopy
Electrophoresis, Polyacrylamide Gel
Fimbriae, Bacterial - classification
Fimbriae, Bacterial - genetics
Fimbriae, Bacterial - ultrastructure
Fimbrial protein
Fundamental and applied biological sciences. Psychology
Microbiology
Microscopy, Electron
Morphology, structure, chemical composition
Pili
Polyclonal antibodies
Protein purification and sequencing
Proteins
Sequence Analysis, DNA
Type 4 fimbria
Western blotting
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Summary:Abstract Type 4 fimbriae have been identified on the cell surface of Actinobacillus pleuropneumoniae by electron microscopy and N-terminal sequencing analysis. A. pleuropneumoniae type 4 fimbrial subunit protein, purified from cell cultures and from outer membrane preparations, reacted with polyclonal antibody raised against type 4 fimbriae of Moraxella bovis on Western blots. N-terminal sequence analysis of the purified 17 kDa type 4 fimbrial subunit protein, named ApfA, revealed the first 12 amino acids to be identical to those of other type 4 fimbrial subunit proteins.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2000.tb09199.x