Cloning and characterisation of a chitin synthase gene cDNA from the cultivated mushroom Agaricus bisporus and its expression during morphogenesis

Abstract Full-length cDNA of a chitin synthase gene (chs1) was cloned from Agaricus bisporus by screening a cDNA library with a PCR amplified fragment of the chitin synthase gene. The chs1 contains an open reading frame of 2727 bp encoding a polypeptide of 909 amino acids and deduced molecular mass...

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Bibliographic Details
Published in:FEMS microbiology letters 2000-08, Vol.189 (1), p.73-77
Main Authors: Sreenivasaprasad, S., Burton, K.S., Wood, D.A.
Format: Article
Language:English
Subjects:
Agaricus - enzymology
Agaricus - genetics
Agaricus - growth & development
Agaricus bisporus
Amino Acid Sequence
Amino acids
Basidiocarps
Biological and medical sciences
Chitin
Chitin synthase
Chitin Synthase - chemistry
Chitin Synthase - genetics
Chitin Synthase - metabolism
Cloning
Cloning, Molecular
DNA, Complementary - genetics
DNA, Fungal - genetics
Domains
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation, Fungal
Genes. Genome
Homology
Microbiology
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Morphogenesis
Mushrooms
Mycelia
Neurospora
Polymerase Chain Reaction
Sequence Analysis, DNA
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Summary:Abstract Full-length cDNA of a chitin synthase gene (chs1) was cloned from Agaricus bisporus by screening a cDNA library with a PCR amplified fragment of the chitin synthase gene. The chs1 contains an open reading frame of 2727 bp encoding a polypeptide of 909 amino acids and deduced molecular mass 102.3 kDa and pI 8.23. The central region of chs1 showed strong homology to other fungal chitin synthase genes with seven conserved domains. It belongs to the chitin synthase class III, analogous to chsB from Aspergillus nidulans, chsC and chsG from A. fumigatus and chs-1 from Neurospora crassa. It appears to be fruit body induced as the transcripts were higher in the developing mushroom compared to any mycelial stage.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2000.tb09208.x