Dopaminergic phenotype of hNT cells in vitro

We investigated the catecholaminergic nature of cultured hNT neurons previously treated either for 4 or 5 weeks with retinoic acid (RA). There were significantly more tyrosine hydroxylase (TH)-positive neurons (60%) in cultures treated for 4 weeks with RA compared to 5 week-treated cultures (≤15%)....

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Bibliographic Details
Published in:Brain research. Developmental brain research 2000-07, Vol.122 (1), p.87-90
Main Authors: Zigova, Tanja, Barroso, Luis F, Willing, Alison E, Saporta, Samuel, McGrogan, Michael P, Freeman, Thomas B, Sanberg, Paul R
Format: Article
Language:English
Subjects:
Aldehyde dehydrogenase
Aldehyde Dehydrogenase - analysis
Antineoplastic Agents - pharmacology
Carrier Proteins - analysis
Cell Line
Dopamine - analysis
Dopamine - genetics
Dopamine Plasma Membrane Transport Proteins
Dopamine receptor D2
Dopamine transporter
hNT neuron
Immunocytochemistry
Immunohistochemistry
In Vitro Techniques
Membrane Glycoproteins
Membrane Transport Proteins
Mesencephalon - cytology
Nerve Tissue Proteins
Neurons - chemistry
Neurons - cytology
Neurons - enzymology
Phenotype
Receptors, Dopamine D2 - analysis
Tretinoin - pharmacology
Tyrosine 3-Monooxygenase - analysis
Tyrosine hydroxylase
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Summary:We investigated the catecholaminergic nature of cultured hNT neurons previously treated either for 4 or 5 weeks with retinoic acid (RA). There were significantly more tyrosine hydroxylase (TH)-positive neurons (60%) in cultures treated for 4 weeks with RA compared to 5 week-treated cultures (≤15%). Furthermore, numerous TH-positive hNT cells were also immunoreactive to dopamine transporter (DAT), dopamine receptor (D2) and aldehyde dehydrogenase (AHD-2), an enzyme exclusively expressed by dopaminergic (DA) ventral mesencephalic (VM) precursors. Thus this cell line has all the necessary cellular machinery to produce functional DA neurons and therefore is a good alternative tissue source to fetal VM.
ISSN:0165-3806
DOI:10.1016/S0165-3806(00)00055-9