Characterization of methanotrophic bacteria on the basis of intact phospholipid profiles

Abstract The intact phospholipid profiles (IPPs) of seven species of methanotrophs from all three physiological groups, type I, II and X, were determined using liquid chromatography/electrospray ionization/mass spectrometry. In these methanotrophs, two major classes of phospholipids were found, phos...

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Bibliographic Details
Published in:FEMS microbiology letters 2000-08, Vol.189 (1), p.67-72
Main Authors: Fang, Jiasong, Barcelona, Michael J., Semrau, Jeremy D.
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Chemotaxonomy
Chromatography, Liquid
Fatty acids
Fundamental and applied biological sciences. Psychology
Ionization
Ions
LC/ESI/MS
Liquid chromatography
Mass spectrometry
Mass Spectrometry - methods
Mass spectroscopy
Methanotroph
Methanotrophic bacteria
Methylocella palustris
Methylococcaceae - chemistry
Methylococcaceae - classification
Methylococcus capsulatus
Methylomicrobium album
Methylomonas methanica
Methylomonas rubra
Methylosinus trichosporium
Microbiology
Morphology, structure, chemical composition
Phosphatidylethanolamine
Phosphatidylglycerol
Phospholipid profile
Phospholipids
Phospholipids - analysis
Principal components analysis
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Summary:Abstract The intact phospholipid profiles (IPPs) of seven species of methanotrophs from all three physiological groups, type I, II and X, were determined using liquid chromatography/electrospray ionization/mass spectrometry. In these methanotrophs, two major classes of phospholipids were found, phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) as well as its derivatives phosphatidylmethylethanolamine (PME) and phosphatidyldimethylethanolamine (PDME). Specifically, the type I methanotrophs, Methylomonas methanica, Methylomonas rubra and Methylomicrobium album BG8 were characterized by PE and PG phospholipids with predominantly C16:1 fatty acids. The type II methanotrophs, Methylosinus trichosporium OB3b and CSC1 were characterized by phospholipids of PG, PME and PDME with predominantly C18:1 fatty acids. Methylococcus capsulatus Bath, a representative of type X methanotrophs, contained mostly PE (89% of the total phospholipids). Finally, the IPPs of a recently isolated acidophilic methanotroph, Methylocella palustris, showed it had a preponderance of PME phospholipids with 18:1 fatty acids (94% of total). Principal component analysis showed these methanotrophs could be clearly distinguished based on phospholipid profiles. Results from this study suggest that IPP can be very useful in bacterial chemotaxonomy.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2000.tb09207.x