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Fertilization and Development of Quail Oocytes after Intracytoplasmic Sperm Injection
The present study was conducted to establish the intracytoplasmic sperm injection (ICSI) method for in vitro fertilization and development in quail. The efficiency of fertilization of oocytes was compared 1) between spontaneous and premature ovulation and 2) among testicular round spermatids, elonga...
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Published in: | Biology of reproduction 2003-11, Vol.69 (5), p.1651-1657 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | The present study was conducted to establish the intracytoplasmic sperm injection (ICSI) method for in vitro fertilization
and development in quail. The efficiency of fertilization of oocytes was compared 1) between spontaneous and premature ovulation
and 2) among testicular round spermatids, elongated spermatids, and immature and mature spermatozoa. The oocytes were injected
with a single spermatozoon or spermatid and cultured for 24 h. Cell division was histologically observed with hematoxylin-eosin
(HE) and a nucleus-specific fluorescent dye (DAPI). Five of 30 (16.6%) and 4 of 30 (13.3%) oocytes injected with mature sperm
were fertilized in the spontaneous and induced ovulation group, respectively. Those embryos showed development at stages IIâVII.
Half the number (three of six) of the oocytes injected with testicular spermatozoa were fertilized and developed to stages
IVâVII, and two of five oocytes injected with elongated spermatids were fertilized and developed to stage VI. All ooocytes
injected with round spermatids were unfertilized. The results demonstrate that intracytoplasmic injection of a single sperm
into quail oocyte can activate the oocyte and lead to fertilization. Oocytes prematurely ovulated are capable of fertilizing
with mature sperm as are those spontaneously ovulated. In addition, the results suggest that the testicular round spermatids
may not possess sufficient oocyte-activating potency but that the elongated spermatids and immature spermatozoa are competent
to participate in fertilization and early embryonic development in quail. |
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ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod.103.019315 |