A population of c-Kit(low)(CD45/TER119)- hepatic cell progenitors of 11-day postcoitus mouse embryo liver reconstitutes cell-depleted liver organoids

Embryo liver morphogenesis takes place after gastrulation and starts with a ventral foregut evagination that reacts to factor signaling from both cardiac mesoderm and septum transversum mesenchyme. Current knowledge of the progenitor stem cell populations involved in this early embryo liver developm...

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Bibliographic Details
Published in:The Journal of clinical investigation 2003-10, Vol.112 (8), p.1152-1163
Main Authors: Minguet, Susana, Cortegano, Isabel, Gonzalo, Pilar, Martínez-Marin, José-Alberto, de Andrés, Belén, Salas, Clara, Melero, David, Gaspar, Maria-Luisa, Marcos, Miguel A R
Format: Article
Language:English
Subjects:
Animals
Bile Ducts - cytology
Cell Differentiation - drug effects
Cell Division - drug effects
Hepatocyte Growth Factor - pharmacology
Hepatocytes - physiology
Keratins - analysis
Leukocyte Common Antigens - physiology
Liver - cytology
Liver - embryology
Liver - ultrastructure
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Oncostatin M
Peptides - pharmacology
Proto-Oncogene Proteins c-kit - physiology
RNA, Messenger - analysis
Stem Cells - physiology
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Summary:Embryo liver morphogenesis takes place after gastrulation and starts with a ventral foregut evagination that reacts to factor signaling from both cardiac mesoderm and septum transversum mesenchyme. Current knowledge of the progenitor stem cell populations involved in this early embryo liver development is scarce. We describe here a population of 11-day postcoitus c-Kit(low)(CD45/TER119)- liver progenitors that selectively expressed hepatospecific genes and proteins in vivo, was self-maintained in vitro by long-term proliferation, and simultaneously differentiated into functional hepatocytes and bile duct cells. Purified c-Kit(low)(CD45/TER119)- liver cells cocultured with cell-depleted fetal liver fragments engrafted and repopulated the hepatic cell compartments of the latter organoids, suggesting that they may include the embryonic stem cells responsible for liver development.
ISSN:0021-9738
DOI:10.1172/JCI17409