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Profiling phenolic metabolites in transgenic alfalfa modified in lignin biosynthesis
Soluble phenolics, wall-bound phenolics and soluble and core lignin were analyzed in transgenic alfalfa with genetically down-regulated O-methyltransferase genes involved in lignin biosynthesis. High performance liquid chromatography and principal component analysis were used to distinguish metaboli...
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| Published in: | Phytochemistry (Oxford) 2003-11, Vol.64 (5), p.1013-1021 |
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| Main Authors: | , , , , |
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| cites | cdi_FETCH-LOGICAL-c446t-951944e4b1cfac9069cf5abdc8b3b1e9e06c656746224b69d4453b5ce5340cc33 |
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| container_title | Phytochemistry (Oxford) |
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| creator | Chen, Fang Duran, Anthony L. Blount, Jack W. Sumner, Lloyd W. Dixon, Richard A. |
| description | Soluble phenolics, wall-bound phenolics and soluble and core lignin were analyzed in transgenic alfalfa with genetically down-regulated
O-methyltransferase genes involved in lignin biosynthesis. High performance liquid chromatography and principal component analysis were used to distinguish metabolic phenotypes of different transgenic alfalfa genotypes growing under standard greenhouse conditions. Principal component analysis of HPLC chromatograms did not resolve differences in leaf metabolite profiles between wild-type and transgenic plants of the same genetic background, although stem phenolic profiles were clearly different between wild-type and transgenic plants. However, the analytical methods clearly differentiated two non-transgenic alfalfa cultivars based on either leaf or stem profiles. Metabolic profiling provides a useful approach to monitoring the broader biochemical phenotypes of transgenic plants with altered expression of lignin pathway enzymes.
Phenylpropanoid metabolism in caffeic acid 3-
O-methyltransferase (COMT) and caffeoyl CoA 3-
O-methyltransferase (CCoAOMT) down-regulated transgenic alfalfa plants were investigated by metabolic profiling approach with statistic analysis. |
| doi_str_mv | 10.1016/S0031-9422(03)00463-1 |
| format | article |
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O-methyltransferase genes involved in lignin biosynthesis. High performance liquid chromatography and principal component analysis were used to distinguish metabolic phenotypes of different transgenic alfalfa genotypes growing under standard greenhouse conditions. Principal component analysis of HPLC chromatograms did not resolve differences in leaf metabolite profiles between wild-type and transgenic plants of the same genetic background, although stem phenolic profiles were clearly different between wild-type and transgenic plants. However, the analytical methods clearly differentiated two non-transgenic alfalfa cultivars based on either leaf or stem profiles. Metabolic profiling provides a useful approach to monitoring the broader biochemical phenotypes of transgenic plants with altered expression of lignin pathway enzymes.
Phenylpropanoid metabolism in caffeic acid 3-
O-methyltransferase (COMT) and caffeoyl CoA 3-
O-methyltransferase (CCoAOMT) down-regulated transgenic alfalfa plants were investigated by metabolic profiling approach with statistic analysis.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/S0031-9422(03)00463-1</identifier><identifier>PMID: 14561519</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Alfalfa ; Biological and medical sciences ; biosynthesis ; Cell Wall - genetics ; Cell Wall - metabolism ; Chromatography, High Pressure Liquid ; Down-Regulation ; enzyme activity ; Fabaceae ; forage legumes ; Fundamental and applied biological sciences. Psychology ; Genotype ; Leguminosae ; Lignin ; Lignin - analysis ; Lignin - biosynthesis ; Medicago sativa ; Medicago sativa - genetics ; Medicago sativa - metabolism ; Metabolic profiling ; Metabolism ; Metabolism. Physicochemical requirements ; methyltransferases ; Methyltransferases - genetics ; Methyltransferases - metabolism ; phenolic compounds ; Phenolics ; Phenols - analysis ; Phenols - metabolism ; Phenotype ; phytochemicals ; plant extracts ; Plant Leaves - genetics ; Plant Leaves - metabolism ; Plant physiology and development ; Plant Stems - genetics ; Plant Stems - metabolism ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Principal Component Analysis ; transgenic plants</subject><ispartof>Phytochemistry (Oxford), 2003-11, Vol.64 (5), p.1013-1021</ispartof><rights>2003 Elsevier Science Ltd</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-951944e4b1cfac9069cf5abdc8b3b1e9e06c656746224b69d4453b5ce5340cc33</citedby><cites>FETCH-LOGICAL-c446t-951944e4b1cfac9069cf5abdc8b3b1e9e06c656746224b69d4453b5ce5340cc33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15200409$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14561519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Fang</creatorcontrib><creatorcontrib>Duran, Anthony L.</creatorcontrib><creatorcontrib>Blount, Jack W.</creatorcontrib><creatorcontrib>Sumner, Lloyd W.</creatorcontrib><creatorcontrib>Dixon, Richard A.</creatorcontrib><title>Profiling phenolic metabolites in transgenic alfalfa modified in lignin biosynthesis</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>Soluble phenolics, wall-bound phenolics and soluble and core lignin were analyzed in transgenic alfalfa with genetically down-regulated
O-methyltransferase genes involved in lignin biosynthesis. High performance liquid chromatography and principal component analysis were used to distinguish metabolic phenotypes of different transgenic alfalfa genotypes growing under standard greenhouse conditions. Principal component analysis of HPLC chromatograms did not resolve differences in leaf metabolite profiles between wild-type and transgenic plants of the same genetic background, although stem phenolic profiles were clearly different between wild-type and transgenic plants. However, the analytical methods clearly differentiated two non-transgenic alfalfa cultivars based on either leaf or stem profiles. Metabolic profiling provides a useful approach to monitoring the broader biochemical phenotypes of transgenic plants with altered expression of lignin pathway enzymes.
Phenylpropanoid metabolism in caffeic acid 3-
O-methyltransferase (COMT) and caffeoyl CoA 3-
O-methyltransferase (CCoAOMT) down-regulated transgenic alfalfa plants were investigated by metabolic profiling approach with statistic analysis.</description><subject>Alfalfa</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>Cell Wall - genetics</subject><subject>Cell Wall - metabolism</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Down-Regulation</subject><subject>enzyme activity</subject><subject>Fabaceae</subject><subject>forage legumes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Leguminosae</subject><subject>Lignin</subject><subject>Lignin - analysis</subject><subject>Lignin - biosynthesis</subject><subject>Medicago sativa</subject><subject>Medicago sativa - genetics</subject><subject>Medicago sativa - metabolism</subject><subject>Metabolic profiling</subject><subject>Metabolism</subject><subject>Metabolism. Physicochemical requirements</subject><subject>methyltransferases</subject><subject>Methyltransferases - genetics</subject><subject>Methyltransferases - metabolism</subject><subject>phenolic compounds</subject><subject>Phenolics</subject><subject>Phenols - analysis</subject><subject>Phenols - metabolism</subject><subject>Phenotype</subject><subject>phytochemicals</subject><subject>plant extracts</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - metabolism</subject><subject>Plant physiology and development</subject><subject>Plant Stems - genetics</subject><subject>Plant Stems - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Principal Component Analysis</subject><subject>transgenic plants</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkF1rFTEQhoMo9rT6E9S9sejF6mTzsbtXIsUvKCi0vQ7J7OxpZDc5JnuE_nuzPQd7WQhMYJ55Z3gYe8XhAweuP14BCF73smnegXgPILWo-RO24V0ratECPGWb_8gJO835NwAopfVzdsKl0lzxfsOuf6U4-smHbbW7pRAnj9VMi3Xlt1CufKiWZEPeUigdO43rq-Y4-NHTsLYnvw2lOB_zXVhuKfv8gj0rXKaXx3rGbr5-ub74Xl_-_Pbj4vNljVLqpe7LBVKSdBxHiz3oHkdl3YCdE45TT6BRK91K3TTS6X6QUgmnkJSQgCjEGTs_5O5S_LOnvJjZZ6RpsoHiPpuWN51SXfcoyNtOlvC2gOoAYoo5JxrNLvnZpjvDwazezb13s0o1IMy9d8PL3Ovjgr2baXiYOoouwNsjYDMWh8Up-vzAqaZEwcq9OXCjjcZuU2FurhrgAjiIoqEpxKcDQcXsX0_JZPQUkAafCBczRP_Isf8ApTmoag</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>Chen, Fang</creator><creator>Duran, Anthony L.</creator><creator>Blount, Jack W.</creator><creator>Sumner, Lloyd W.</creator><creator>Dixon, Richard A.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>Profiling phenolic metabolites in transgenic alfalfa modified in lignin biosynthesis</title><author>Chen, Fang ; Duran, Anthony L. ; Blount, Jack W. ; Sumner, Lloyd W. ; Dixon, Richard A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-951944e4b1cfac9069cf5abdc8b3b1e9e06c656746224b69d4453b5ce5340cc33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Alfalfa</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>Cell Wall - genetics</topic><topic>Cell Wall - metabolism</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Down-Regulation</topic><topic>enzyme activity</topic><topic>Fabaceae</topic><topic>forage legumes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Leguminosae</topic><topic>Lignin</topic><topic>Lignin - analysis</topic><topic>Lignin - biosynthesis</topic><topic>Medicago sativa</topic><topic>Medicago sativa - genetics</topic><topic>Medicago sativa - metabolism</topic><topic>Metabolic profiling</topic><topic>Metabolism</topic><topic>Metabolism. Physicochemical requirements</topic><topic>methyltransferases</topic><topic>Methyltransferases - genetics</topic><topic>Methyltransferases - metabolism</topic><topic>phenolic compounds</topic><topic>Phenolics</topic><topic>Phenols - analysis</topic><topic>Phenols - metabolism</topic><topic>Phenotype</topic><topic>phytochemicals</topic><topic>plant extracts</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - metabolism</topic><topic>Plant physiology and development</topic><topic>Plant Stems - genetics</topic><topic>Plant Stems - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Principal Component Analysis</topic><topic>transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Fang</creatorcontrib><creatorcontrib>Duran, Anthony L.</creatorcontrib><creatorcontrib>Blount, Jack W.</creatorcontrib><creatorcontrib>Sumner, Lloyd W.</creatorcontrib><creatorcontrib>Dixon, Richard A.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Fang</au><au>Duran, Anthony L.</au><au>Blount, Jack W.</au><au>Sumner, Lloyd W.</au><au>Dixon, Richard A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Profiling phenolic metabolites in transgenic alfalfa modified in lignin biosynthesis</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>64</volume><issue>5</issue><spage>1013</spage><epage>1021</epage><pages>1013-1021</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>Soluble phenolics, wall-bound phenolics and soluble and core lignin were analyzed in transgenic alfalfa with genetically down-regulated
O-methyltransferase genes involved in lignin biosynthesis. High performance liquid chromatography and principal component analysis were used to distinguish metabolic phenotypes of different transgenic alfalfa genotypes growing under standard greenhouse conditions. Principal component analysis of HPLC chromatograms did not resolve differences in leaf metabolite profiles between wild-type and transgenic plants of the same genetic background, although stem phenolic profiles were clearly different between wild-type and transgenic plants. However, the analytical methods clearly differentiated two non-transgenic alfalfa cultivars based on either leaf or stem profiles. Metabolic profiling provides a useful approach to monitoring the broader biochemical phenotypes of transgenic plants with altered expression of lignin pathway enzymes.
Phenylpropanoid metabolism in caffeic acid 3-
O-methyltransferase (COMT) and caffeoyl CoA 3-
O-methyltransferase (CCoAOMT) down-regulated transgenic alfalfa plants were investigated by metabolic profiling approach with statistic analysis.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>14561519</pmid><doi>10.1016/S0031-9422(03)00463-1</doi><tpages>9</tpages></addata></record> |
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| source | Elsevier |
| subjects | Alfalfa Biological and medical sciences biosynthesis Cell Wall - genetics Cell Wall - metabolism Chromatography, High Pressure Liquid Down-Regulation enzyme activity Fabaceae forage legumes Fundamental and applied biological sciences. Psychology Genotype Leguminosae Lignin Lignin - analysis Lignin - biosynthesis Medicago sativa Medicago sativa - genetics Medicago sativa - metabolism Metabolic profiling Metabolism Metabolism. Physicochemical requirements methyltransferases Methyltransferases - genetics Methyltransferases - metabolism phenolic compounds Phenolics Phenols - analysis Phenols - metabolism Phenotype phytochemicals plant extracts Plant Leaves - genetics Plant Leaves - metabolism Plant physiology and development Plant Stems - genetics Plant Stems - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Principal Component Analysis transgenic plants |
| title | Profiling phenolic metabolites in transgenic alfalfa modified in lignin biosynthesis |
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