Cloning, tissue expression pattern, and chromosome location of a novel human gene BRI3BP

• Published in: Biochemical genetics 2001-12, Vol.39 (11-12), p.369-377
• Main Authors: Lin, L, Wu, Y, Li, C, Zhao, S
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino acids; Apoptosis; Baits; Base Sequence; Brain; BRI3 gene; BRI3BP gene; Carrier Proteins; Chromosome 12; Chromosome Mapping; Chromosomes; Chromosomes, Human, Pair 12; Cloning; Cloning, Molecular; Coding; DNA, Complementary; Fetuses; Gene Expression; Gene Expression Profiling; Gene mapping; Genes; Genetics; Humans; Hybridization; Isoelectric points; Kidney; Liver; Membrane Proteins - biosynthesis; Membrane Proteins - genetics; Membrane Proteins - physiology; Molecular Sequence Data; Molecular weight; mRNA; Nerve Tissue Proteins; Open reading frames; Polymerase Chain Reaction; Sequence Alignment; Tissue Distribution; Yeasts
• ISSN: 0006-2928; 1573-4927
• DOI: 10.1023/A:1013807518791

A novel cDNA fragment was identified from a human fetal brain cDNA library by using the coding sequence of human BRI3 gene (Accession No. NM015379) as bait in a yeast two-hybrid screening. Then by 5'-RACE (rapid amplification of cDNA end) and electronic hybridization, we obtained a 1.9 kb contig which consists of a novel gene. It was designated as BRI3BP by the HUGO Nomenclature Committee. It contains an open reading frame encoding 251 amino acids. The calculated molecular weight of the deduced protein is 27.8 kU. The predicted isoelectric point is 9.48. Northern hybridization showed its mRNA was highly expressed in brain, kidney, and liver. By RH mapping, the BRI3BP gene was mapped to human chromosome 12q24.2-qter