Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation

• Published in: Intensive care medicine 2003-10, Vol.29 (10), p.1782-1789
• Main Authors: RITTER, Cristiane, ANDRADES, Michael, DAL-PIZZOL, Felipe, FROTA, Mario Luis C, BONATTO, Fernanda, PINHO, Ricardo A, POLYDORO, Manuela, KLAMT, Fabio, PINHEIRO, Cleovaldo T. S, MENNA-BARRETO, Sérgio S, MOREIRA, José Claudio F
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Bacterial diseases; Bacterial sepsis; Biological and medical sciences; Cecal Diseases - complications; Cecum; Free radicals; Human bacterial diseases; Infectious diseases; Intensive care; Intestinal Perforation - complications; Laparotomy; Ligation; Lipid peroxidation; Male; Medical sciences; Mortality; Oxidative Stress; Prospective Studies; Random Allocation; Rats; Rats, Wistar; Sepsis; Sepsis - etiology; Sepsis - metabolism; Sepsis - mortality; Tumor necrosis factor-TNF
• ISSN: 0342-4642; 1432-1238
• DOI: 10.1007/s00134-003-1789-9

This study assessed parameters of free radical damage to biomolecules, mitochondrial superoxide production, superoxide dismutase, and catalase activities and their relationship to sepsis mortality. Prospective animal study in a university laboratory for experimental. 140 male Wistar rats. The animals were randomly divided into three groups: sham-operated (n=20), cecal ligation and perforation resuscitated with normal saline (n=40), and cecal ligation and perforation with normal saline plus antibiotics (n=40). Blood samples were collected from all animals 3, 12, and 24 h after CLP through a jugular catheter inserted before CLP. Rats were evaluated during 5 days after the intervention. Nonsurvivor animals were grouped according to the duration between sepsis induction and death, and oxidative parameters were compared to survivors and sham-operated. Lipid peroxidation, protein carbonyls, and superoxide dismutase were significantly increased in nonsurvivor septic rats and were predictive of mortality. We demonstrated that there is a different modulation of superoxide dismutase and catalase in nonsurvivors during the course of septic response. There was a marked increase in superoxide dismutase activity without a proportional increase in catalase activity in nonsurvivors. This is the first report of plasma superoxide dismutase as an earlier marker of mortality. Ours results might help to clarify an important aspect of oxidative response to sepsis, i.e., an increase in superoxide dismutase activity without a proportional increase in catalase activity