Comparative evaluation of a commercial test for rapid identification of methicillin-resistant Staphylococcus aureus

The performance and ease of use of the recently introduced MRSA screen test (Denka Seiken Co. Ltd., Japan) for the identification of methicillin‐resistant Staphylococcus aureus was evaluated in comparison with the BBL Crystal MRSA ID System (Becton Dickinson Europe, France). A total of 109 strains o...

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Bibliographic Details
Published in:APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2001-11, Vol.109 (11), p.787-790
Main Authors: JUREEN, R., BOTTOLFSEN, K. LILAND, GREWAL, H., DIGRANES, A.
Format: Article
Language:English
Subjects:
agglutination test
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bacterial Proteins
Bacteriological methods and techniques used in bacteriology
Bacteriological Techniques
Bacteriology
Biological and medical sciences
Carrier Proteins - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Hexosyltransferases
Humans
Medical sciences
Methicillin Resistance - genetics
Microbial Sensitivity Tests - methods
Microbiology
MRSA
Muramoylpentapeptide Carboxypeptidase - genetics
PBP2a
Penicillin-Binding Proteins
Peptidyl Transferases
Pharmacology. Drug treatments
Polymerase Chain Reaction
Staphylococcus aureus - drug effects
Staphylococcus aureus - genetics
Staphylococcus aureus - isolation & purification
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Summary:The performance and ease of use of the recently introduced MRSA screen test (Denka Seiken Co. Ltd., Japan) for the identification of methicillin‐resistant Staphylococcus aureus was evaluated in comparison with the BBL Crystal MRSA ID System (Becton Dickinson Europe, France). A total of 109 strains of S. aureus, consisting of 57 strains of MecA‐negative S. aureus and 52 strains of MecA‐positive S. aureus, were tested. With MecA PCR as the gold standard, the MRSA screen test had 98% sensitivity and 98% specificity, whereas the BBL Crystal MRSA ID System had 98% sensitivity and 95% specificity. The simplicity of use and rapid result make the MRSA screen test a valuable tool in the clinical microbiology laboratory pending demonstration of the MecA gene that should still always be done.
ISSN:0903-4641
1600-0463
DOI:10.1034/j.1600-0463.2001.d01-147.x