Characterization of eight Y-STR loci and haplotypes in a Chinese Han population

In this study we analyzed the eight Y-STR loci, DYS443, DYS444, DYS448, DYS453, DYS455, DYS456, DYS457 (DYS437) and DYS458, investigated haplotype distributions of these Y-STR loci in a Chinese Han population, and sequenced alleles of the eight loci for clarifying the structure. Extracted DNA was am...

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Bibliographic Details
Published in:International journal of legal medicine 2003-10, Vol.117 (5), p.263-270
Main Authors: Tang, Jian Pin, Hou, Yi Ping, Li, Ying Bi, Wu, Jin, Zhang, Jian, Zhang, Hai Jun
Format: Article
Language:English
Subjects:
Alleles
Asian Continental Ancestry Group - genetics
China
Chromosomes, Human, Y
Cloning
Female
Forensic Medicine - methods
Forensic sciences
Genetics, Population
Haplotypes
Humans
Male
Polymerase Chain Reaction
Tandem Repeat Sequences
Y chromosomes
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Summary:In this study we analyzed the eight Y-STR loci, DYS443, DYS444, DYS448, DYS453, DYS455, DYS456, DYS457 (DYS437) and DYS458, investigated haplotype distributions of these Y-STR loci in a Chinese Han population, and sequenced alleles of the eight loci for clarifying the structure. Extracted DNA was amplified by PCR and the PCR products were analyzed by non-denaturing horizontal polyacrylamide gel electrophoresis with a discontinuous buffer system. Alleles were sequenced on an ABI 3700 using a Dye Terminator Cycle sequencing kit. DYS443, DYS453, DYS455 and DYS456 were found to be simple repeat systems, while DYS444, DYS448, DYS457 (DYS437) and DYS458 were complex repeat systems. The gene diversities of DYS443, DYS444, DYS448, DYS453, DYS455, DYS456, DYS457 (DYS437) and DYS458 were 0.7742, 0.7671, 0.7453, 0.3545, 0.0549, 0.6988, 0.6148 and 0.8213, respectively. The haplotype diversity for 8 Y-STR loci was 0.9996, and the discrimination capacity was 0.9815. The results indicate that these eight loci are useful Y-linked markers for forensic applications.
ISSN:0937-9827
1437-1596
DOI:10.1007/s00414-003-0371-x