Red blood cells promote survival and cell cycle progression of human peripheral blood T cells independently of CD58/LFA-3 and heme compounds

Red blood cells (RBC) are known to modulate T cell proliferation and function possibly through downregulation of oxidative stress. By examining parameters of activation, division, and cell death in vitro, we show evidence that the increase in survival afforded by RBC is due to the maintenance of the...

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Bibliographic Details
Published in:Cellular immunology 2003-07, Vol.224 (1), p.17-28
Main Authors: Fonseca, Ana Mafalda, Pereira, Carlos Filipe, Porto, Graça, Arosa, Fernando A.
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - physiology
CD2 Antigens - immunology
CD2 Antigens - metabolism
CD3 Complex - physiology
CD58 Antigens - immunology
CD58 Antigens - metabolism
CD58/LFA-3
CD8
CD8-Positive T-Lymphocytes - cytology
CD8-Positive T-Lymphocytes - metabolism
Cell Communication - physiology
Cell cycle
Cell Cycle - physiology
Cell Survival - physiology
Cells, Cultured
Erythrocytes - cytology
Erythrocytes - metabolism
Heme
Heme - metabolism
Heme - pharmacology
Humans
Lymphocyte Activation - drug effects
Lymphocyte Activation - immunology
Red blood cell
Survival
T cell
T-Lymphocytes - cytology
T-Lymphocytes - metabolism
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Summary:Red blood cells (RBC) are known to modulate T cell proliferation and function possibly through downregulation of oxidative stress. By examining parameters of activation, division, and cell death in vitro, we show evidence that the increase in survival afforded by RBC is due to the maintenance of the proliferative capacity of the activated T cells. We also show that the CD3+CD8+ T cell subset was preferentially expanded and rescued from apoptosis both in bulk peripheral blood lymphocyte cultures and with highly purified CD8+ T cells. The ability of RBC to induce survival of dividing T cells was not affected by blocking the CD58/CD2 interaction. Moreover, addition of hemoglobin, heme or protoporphyrin IX to cultures of activated T cells did not reproduce the effect of intact RBC. Considering that RBC circulate throughout the body, they could play a biological role in the modulation of T cell differentiation and survival in places of active cell division. Neither CD58 nor the heme compounds studied seem to play a direct relevant role in the modulation of T cell survival.
ISSN:0008-8749
1090-2163
DOI:10.1016/S0008-8749(03)00170-9