Patterning of photosensitive polyimide LB film and its application in the fabrication of biomolecular microphotodiode array

• Published in: Biosensors & bioelectronics 2003-11, Vol.19 (2), p.103-108
• Main Authors: Oh, Se Young, Park, Joon-Kyu, Ko, Chang-Bum, Choi, Jeong-Woo
• Format: Article
• Language: English
• Subjects: 11-mercaptoundecanoic acid; Adsorption; Biological and medical sciences; Biomimetic Materials - chemistry; Biomimetic Materials - radiation effects; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensors; Biotechnology; Coated Materials, Biocompatible - chemical synthesis; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - radiation effects; Cytochrome c; Cytochromes c - chemistry; Cytochromes c - radiation effects; Electric Conductivity; Electrochemistry - instrumentation; Electrochemistry - methods; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - radiation effects; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; GFP; Light; Manufactured Materials; Membranes, Artificial; Methods. Procedures. Technologies; Micro array pattern; Microelectrodes; Miniaturization; Photochemistry - instrumentation; Photochemistry - methods; Photodiode; polyimide; Polyimide LB film; Redox activity; Reproducibility of Results; Resins, Synthetic; Self-assembly process; Semiconductors; Sensitivity and Specificity; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/S0956-5663(03)00177-5

Ultra thin film of photosensitive polyimide having benzene and sulfonyloxyimide moieties in the main chain was prepared using a Langmuir–Blodgett (LB) technique, and then micro array pattern of the polyimide LB film on a gold substrate was obtained by deep UV lithographic technique. In order to array cytochrome c molecules along the micro-patterned gold substrate, the well-characterized monolayer of cytochrome c was immobilized with a mixed monolayer of 11-mercaptoundecanoic acid (11-MUDA) and decanethiol. The redox activity and electron transfer between cytochrome c molecular center and gold electrode interface for the self-assembled cytochrome c monolayer were investigated by cyclic voltammetry measurement. Biomolecular photodiode consisting of cytochrome c and green fluorescent protein (GFP) onto the patterned gold substrate was fabricated by self-assembly process. The integration and morphology of cytochrome c and GFP were studied from the measurements of atomic force microscopy (AFM) and fluorescence emission. Especially, current–voltage characteristics of the protein multilayers were investigated by scanning tunneling microscopy (STM) and its application in biomolecular photodiode was also examined.