Unchanged G-protein-coupled receptor kinase activity in the aging human heart

We sought to find out whether G-protein-coupled receptor kinase (GRK) activity is also increased in the aging human heart. In the aging and failing human heart, cardiac beta-adrenoceptors (beta-AR) are desensitized. In heart failure (HF), an increase in cardiac GRK activity considerably contributes...

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Bibliographic Details
Published in:Journal of the American College of Cardiology 2003-10, Vol.42 (8), p.1487-1492
Main Authors: Leineweber, Kirsten, Klapproth, Stefan, Beilfuß, Anja, Silber, Rolf-Edgar, Heusch, Gerd, Philipp, Thomas, Brodde, Otto-Erich
Format: Article
Language:English
Subjects:
Adenylyl Cyclases - metabolism
Adult
Age
Aged
Aging - metabolism
Atrial Appendage - enzymology
beta-Adrenergic Receptor Kinases
Biological and medical sciences
Cardiology
Cardiology. Vascular system
Cardiovascular disease
Child
Consent
Cyclic AMP-Dependent Protein Kinases - metabolism
Cyclic AMP-Dependent Protein Kinases - physiology
Female
Heart
Heart Failure - enzymology
Heart failure, cardiogenic pulmonary edema, cardiac enlargement
Humans
Kinases
Male
Males
Medical sciences
Middle Aged
Myocardium - enzymology
Nitrogen
Proteins
Surgery
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Summary:We sought to find out whether G-protein-coupled receptor kinase (GRK) activity is also increased in the aging human heart. In the aging and failing human heart, cardiac beta-adrenoceptors (beta-AR) are desensitized. In heart failure (HF), an increase in cardiac GRK activity considerably contributes to this beta-AR desensitization. We assessed GRK activity (by in vitro rhodopsin phosphorylation) in the right atria (RA) from 16 children (mean age 9 ± 2 years) and 17 elderly patients (mean age 67 ± 2 years) without apparent HF and in the RA from four patients with end-stage HF. Cytosolic and membranous GRK activities in the RA from children were not significantly different from those in elderly patients; in contrast, cytosolic and membranous GRK activities in the RA from patients with end-stage HF were significantly increased. In contrast to the failing human heart, in the aging human heart, GRK activity is not increased. Thus, GRK activity appears to not play an important role in beta-AR desensitization in the aging human heart.
ISSN:0735-1097
1558-3597
DOI:10.1016/S0735-1097(03)01063-5