Cytochrome P450 2A6: a new hepatic autoantigen in patients with chronic hepatitis C virus infection

• Published in: Journal of hepatology 2003-11, Vol.39 (5), p.800-806
• Main Authors: Dalekos, Georgios N, Obermayer-Straub, Petra, Bartels, Moritz, Maeda, Takashi, Kayser, Anne, Braun, Sabine, Loges, Stephanie, Schmidt, Eleonore, Gershwin, M.Eric, Manns, Michael P
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aryl Hydrocarbon Hydroxylases - immunology; Autoantibodies - analysis; Autoantigens - immunology; Autoimmune Diseases - immunology; Autoimmune hepatitis; Autoimmunity; Case-Control Studies; Child; Chronic Disease; Chronic hepatitis; Cytochrome P-450 CYP2A6; Cytochrome P450 1A2; Cytochrome P450 2A6; Cytochrome P450 2D6; Female; Flaviviridae Infections - immunology; Hepatitis C virus; Hepatitis C, Chronic - immunology; Hepatitis, Viral, Human - immunology; Humans; Liver Diseases - immunology; LKM antibody; Male; Middle Aged; Mixed Function Oxygenases - immunology; Rheumatic Diseases - immunology
• ISSN: 0168-8278; 1600-0641
• DOI: 10.1016/S0168-8278(03)00356-8

Background/Aims : Cytochromes P4502A6 (CYP2A6) and P4501A2 (CYP1A2) were described as hepatic autoantigens in the autoimmune polyglandular syndrome type-1 (APS-1). We evaluated the significance of anti-CYP2A6 and anti-CYP1A2 in several hepatic diseases in the absence of APS-1. Methods : A radioligand assay (RLA) based on immunoprecipitation of [ 35S]-methionine-labeled CYP2A6 and CYP1A2 was used. Four hundred and thirty subjects with chronic viral hepatitis ( n=185), autoimmune liver diseases ( n=181), autoimmune rheumatic diseases (ARD, n=31) and healthy ( n=33) were tested. Results : Seven out of 366 patients with liver diseases were anti-CYP2A6 positive. Neither healthy nor ARD patients showed anti-CYP2A6. One out of 181 patients with autoimmune liver diseases tested anti-CYP2A6 positive. A significantly higher prevalence of anti-CYP2A6 ( P