Stenotrophomonas maltophilia contamination of nebulizers used to deliver aerosolized therapy to inpatients with cystic fibrosis

There is circumstantial evidence that nebulizer equipment may be a source of Stenotrophomonas maltophilia for patients with cystic fibrosis. Eighty-nine inpatient nebulizers were examined for evidence of S. maltophilia contamination of which nine (10%) yielded 14 strains of the bacterium. Environmen...

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Bibliographic Details
Published in:The Journal of hospital infection 2003-11, Vol.55 (3), p.180-183
Main Authors: Denton, M, Rajgopal, A, Mooney, L, Qureshi, A, Kerr, K.G, Keer, V, Pollard, K, Peckham, D.G, Conway, S.P
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aerosols
Biological and medical sciences
Contamination
Cystic fibrosis
Cystic Fibrosis - drug therapy
Electrophoresis, Gel, Pulsed-Field
Equipment Contamination
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
Nebulizers
Nebulizers and Vaporizers - microbiology
Other diseases. Semiology
pulsed-field gel electrophoresis
Stenotrophomonas maltophilia
Stenotrophomonas maltophilia - genetics
Stenotrophomonas maltophilia - isolation & purification
Water Microbiology
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Summary:There is circumstantial evidence that nebulizer equipment may be a source of Stenotrophomonas maltophilia for patients with cystic fibrosis. Eighty-nine inpatient nebulizers were examined for evidence of S. maltophilia contamination of which nine (10%) yielded 14 strains of the bacterium. Environmental samples were obtained from 73 different sites on the ward, of which 17 (23%) yielded a further 21 strains. Positive sites included taps, sink drains, and potable water. Genotyping using ERIC-PCR and pulsed-field gel electrophoresis revealed that two pairs of patients' nebulizers were contaminated with closely related strains. None of the S. maltophilia isolates obtained from the ward environment shared genotypes with those obtained from the nebulizers. The frequency of isolation of S. maltophilia from potable water sources on the ward suggests that contamination may result from using it to clean reusable nebulizer equipment, particularly if this is followed by inadequate drying. Although the actual source of S. maltophilia contamination of hospital-use nebulizer equipment in this study remained elusive, these results have important infection control implications.
ISSN:0195-6701
1532-2939
DOI:10.1016/S0195-6701(03)00299-8