Imaging of Membrane Lipids in Single Cells by Imprint-Imaging Time-of-Flight Secondary Ion Mass Spectrometry

A new method for identification and localization of organic molecules in biological samples is described. The method involves making an imprint of a biological sample on a silver (Ag) surface and subsequent analysis of the imprint by imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS)....

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2003-07, Vol.75 (14), p.3429-3434
Main Authors: Sjövall, Peter, Lausmaa, Jukka, Nygren, Håkan, Carlsson, Lennart, Malmberg, Per
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical chemistry
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Cells - chemistry
Chemistry
Diagnostic Imaging
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Humans
Mass Spectrometry
Membrane Lipids - chemistry
Membranes - chemistry
Membranes - ultrastructure
Microscopy, Electron, Scanning
Spectrometric and optical methods
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Summary:A new method for identification and localization of organic molecules in biological samples is described. The method involves making an imprint of a biological sample on a silver (Ag) surface and subsequent analysis of the imprint by imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS). Using this method, detection of unfragmented, Ag cationized molecules at a spatial resolution of
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0207675