Temporal and spatial assembly of lipid droplet-associated proteins in 3T3-L1 preadipocytes

This study aimed to investigate the relationship between newly formed lipid droplets and lipid droplet surface proteins, including perilipin, adipose differentiation related protein (ADRP), and p200 kDa protein (p200) in 3T3-L1 preadipocytes, during lipogenesis. Sterol ester was used to induce nasce...

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Bibliographic Details
Published in:Histochemistry and cell biology 2003-10, Vol.120 (4), p.285-292
Main Authors: Wang, Seu-Mei, Hwang, Ran-Der, Greenberg, Andrew S, Yeo, Hui-Ling
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Adipocytes - chemistry
Adipocytes - metabolism
Adipocytes - ultrastructure
Animals
Carrier Proteins
Lipids - biosynthesis
Membrane Proteins - analysis
Membrane Proteins - metabolism
Mice
Perilipin-1
Perilipin-2
Phosphoproteins - analysis
Phosphoproteins - metabolism
Stem Cells - chemistry
Stem Cells - metabolism
Stem Cells - ultrastructure
Time Factors
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Summary:This study aimed to investigate the relationship between newly formed lipid droplets and lipid droplet surface proteins, including perilipin, adipose differentiation related protein (ADRP), and p200 kDa protein (p200) in 3T3-L1 preadipocytes, during lipogenesis. Sterol ester was used to induce nascent lipid droplets in 3T3-L1 preadipocytes and the sequence of lipids and lipid droplet surface proteins was studied using a combination of immunohistochemistry and Nile red staining/Oil red O. We demonstrated that, although most growing lipid droplets appeared to have a lipid core surrounded by a fluorescent rim of ADRP, perilipin, and p200, tiny protein aggregates of ADRP, perilipin, or p200 could also be found to occur in the absence of lipid accumulation. In addition, ADRP associated with nascent lipid droplets prior to that of perilipin or p200. We provide evidence that lipid droplet surface proteins, especially ADRP and perilipin, are important in serving as a nucleation center for the assembly of lipid to form nascent lipid droplets.
ISSN:0948-6143
1432-119X
DOI:10.1007/s00418-003-0575-7