Human Kv1.6 current displays a C-type-like inactivation when re-expressed in cos-7 cells

The human Kv1.6 K + channel was functionally re-expressed in COS-7 cells at different levels. Voltage-activated K + currents are recorded upon cell membrane depolarization independently of the level of Kv1.6 expression. The current acquires a fast inactivation when Kv1.6 expression is increased. Ina...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2003-11, Vol.311 (1), p.83-89
Main Authors: Guihard, G, Bellocq, C, Grelet, E, Escande, D
Format: Article
Language:English
Subjects:
Action potential
Adaptation, Physiological - drug effects
Adaptation, Physiological - physiology
Animals
Cercopithecus aethiops
COS Cells
Delayed Rectifier Potassium Channels
Electric Conductivity
Glial cell
Humans
Ion channel
Ion Channel Gating - drug effects
Ion Channel Gating - genetics
Ion Channel Gating - physiology
Membrane Potentials - drug effects
Membrane Potentials - physiology
Neuron
Patch-clamp
Potassium Channels - drug effects
Potassium Channels - genetics
Potassium Channels - physiology
Potassium Channels, Voltage-Gated
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Repolarization
Tetraethylammonium - pharmacology
Transfection - methods
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Summary:The human Kv1.6 K + channel was functionally re-expressed in COS-7 cells at different levels. Voltage-activated K + currents are recorded upon cell membrane depolarization independently of the level of Kv1.6 expression. The current acquires a fast inactivation when Kv1.6 expression is increased. Inactivation was not affected by divalent cations or by extracellular tetraethylammonium. We have characterized the inactivation properties in biophysical terms. The fraction of inactivated current and the kinetics of inactivation are increased as the cell becomes more depolarized. Inactivated current can be reactivated according to a bi-exponential function of time. Additional experiments indicate that Kv1.6 inactivation properties are close to those of a conventional C-type inactivation. This work suggests that the concentration of Kv1.6 channel in the cell membrane strongly modulates the kinetic properties of Kv1.6-induced K + current. The physiological implications of these modifications are discussed.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2003.09.167