Loading…
Carboxy-terminally truncated Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induced neutralizing antibodies and resistance to Dengue 4 virus challenge in mice
We have expressed a recombinant Dengue 4 virus envelope glycoprotein (E4rec), truncated at its C-terminus by 53 amino acids, in Pichia pastoris. The presence of E4rec was confirmed by Western-blot using anti-DEN 4 hyper immune mouse ascitic fluid. E4rec migrated during SDS-PAGE as a 64 kDa protein....
Saved in:
Published in: | Archives of virology 2003-11, Vol.148 (11), p.2267-2273 |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c428t-2ee8cd35d392e0af77cfd47a30455882881e2169bd09b47fe08522f0ede91b723 |
---|---|
cites | |
container_end_page | 2273 |
container_issue | 11 |
container_start_page | 2267 |
container_title | Archives of virology |
container_volume | 148 |
creator | MUNE, M RODRIGUEZ, R RAMIREZ, R SOTO, Y SIERRA, B RODRIGUEZ ROCHE, R MARQUEZ, G GARCIA, J GUILLEN, G GUZMAN, M. G |
description | We have expressed a recombinant Dengue 4 virus envelope glycoprotein (E4rec), truncated at its C-terminus by 53 amino acids, in Pichia pastoris. The presence of E4rec was confirmed by Western-blot using anti-DEN 4 hyper immune mouse ascitic fluid. E4rec migrated during SDS-PAGE as a 64 kDa protein. Treatment with endoglycosidases showed that the E protein was modified by the addition of short mannose chains and the absence of hyperglycosylation. When administered to BALB-C mice, E4rec elicited a DEN 4 neutralizing antibody response haemagglutination inhibition antibodies and specific memory T cell response. Mice immunized were also significantly protected against lethal DEN 4 virus challenge (86.6%, p < 0.001). |
doi_str_mv | 10.1007/s00705-003-0167-9 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71309947</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18888643</sourcerecordid><originalsourceid>FETCH-LOGICAL-c428t-2ee8cd35d392e0af77cfd47a30455882881e2169bd09b47fe08522f0ede91b723</originalsourceid><addsrcrecordid>eNqFkcuOFCEUhonROO3oA7gxxER3pdyqgKVpr8kkutA1oeBUD5MqaIGaTPtWvqF0upNJZiMLOBy-_wDnR-glJe8oIfJ9aRPpO0J4R-ggO_0IbajgrFNSq8doQzgRnRqIukDPSrkhDSS8f4ouqOilpopv0N-tzWO6O3QV8hKinecDrnmNzlbw-CPE3QpY4NuQ14Ih3sKc9oB388GlfU4VQsRwt89QSsPb5kdw18HivS015VBayq-uHUVYa7Zz-BPiDttYw5h8gNJCj5s6lGqjA1zTwzvddXtTS8Gx-hIcPEdPJjsXeHFeL9Gvz59-br92V9-_fNt-uOqcYKp2DEA5z3vPNQNiJynd5IW0rSV9rxRTigKjgx490aOQExDVMzYR8KDpKBm_RG9Pdds_f69QqllCcTDPNkJai5GUE62F_C9IVRuD4A18_QC8SWtuPS-GUSYIG8TQIHqCXE6lZJjMPofF5oOhxBxdNyfXTTPTHF03umlenQuv4wL-XnG2uQFvzoAtzs5Tbs0O5Z7rmaJKD_wf8Xm4XA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>212402646</pqid></control><display><type>article</type><title>Carboxy-terminally truncated Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induced neutralizing antibodies and resistance to Dengue 4 virus challenge in mice</title><source>Springer Nature</source><creator>MUNE, M ; RODRIGUEZ, R ; RAMIREZ, R ; SOTO, Y ; SIERRA, B ; RODRIGUEZ ROCHE, R ; MARQUEZ, G ; GARCIA, J ; GUILLEN, G ; GUZMAN, M. G</creator><creatorcontrib>MUNE, M ; RODRIGUEZ, R ; RAMIREZ, R ; SOTO, Y ; SIERRA, B ; RODRIGUEZ ROCHE, R ; MARQUEZ, G ; GARCIA, J ; GUILLEN, G ; GUZMAN, M. G</creatorcontrib><description>We have expressed a recombinant Dengue 4 virus envelope glycoprotein (E4rec), truncated at its C-terminus by 53 amino acids, in Pichia pastoris. The presence of E4rec was confirmed by Western-blot using anti-DEN 4 hyper immune mouse ascitic fluid. E4rec migrated during SDS-PAGE as a 64 kDa protein. Treatment with endoglycosidases showed that the E protein was modified by the addition of short mannose chains and the absence of hyperglycosylation. When administered to BALB-C mice, E4rec elicited a DEN 4 neutralizing antibody response haemagglutination inhibition antibodies and specific memory T cell response. Mice immunized were also significantly protected against lethal DEN 4 virus challenge (86.6%, p < 0.001).</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-003-0167-9</identifier><identifier>PMID: 14579183</identifier><language>eng</language><publisher>Wien: Springer</publisher><subject>Amino acids ; Animals ; Antibodies ; Antibodies, Viral - blood ; Biological and medical sciences ; Dengue - prevention & control ; Dengue fever ; Dengue Virus - immunology ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genomes ; Glycoproteins ; Human viral diseases ; Immunization ; Infectious diseases ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Microbiology ; Miscellaneous ; Mycology ; Neutralization Tests ; Pichia - genetics ; Proteins ; Recombinant Proteins - immunology ; Vaccines, Synthetic - immunology ; Viral diseases ; Viral Envelope Proteins - immunology ; Viral Vaccines - immunology ; Virology ; Viruses ; Yeast</subject><ispartof>Archives of virology, 2003-11, Vol.148 (11), p.2267-2273</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-2ee8cd35d392e0af77cfd47a30455882881e2169bd09b47fe08522f0ede91b723</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15281896$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14579183$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MUNE, M</creatorcontrib><creatorcontrib>RODRIGUEZ, R</creatorcontrib><creatorcontrib>RAMIREZ, R</creatorcontrib><creatorcontrib>SOTO, Y</creatorcontrib><creatorcontrib>SIERRA, B</creatorcontrib><creatorcontrib>RODRIGUEZ ROCHE, R</creatorcontrib><creatorcontrib>MARQUEZ, G</creatorcontrib><creatorcontrib>GARCIA, J</creatorcontrib><creatorcontrib>GUILLEN, G</creatorcontrib><creatorcontrib>GUZMAN, M. G</creatorcontrib><title>Carboxy-terminally truncated Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induced neutralizing antibodies and resistance to Dengue 4 virus challenge in mice</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><description>We have expressed a recombinant Dengue 4 virus envelope glycoprotein (E4rec), truncated at its C-terminus by 53 amino acids, in Pichia pastoris. The presence of E4rec was confirmed by Western-blot using anti-DEN 4 hyper immune mouse ascitic fluid. E4rec migrated during SDS-PAGE as a 64 kDa protein. Treatment with endoglycosidases showed that the E protein was modified by the addition of short mannose chains and the absence of hyperglycosylation. When administered to BALB-C mice, E4rec elicited a DEN 4 neutralizing antibody response haemagglutination inhibition antibodies and specific memory T cell response. Mice immunized were also significantly protected against lethal DEN 4 virus challenge (86.6%, p < 0.001).</description><subject>Amino acids</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - blood</subject><subject>Biological and medical sciences</subject><subject>Dengue - prevention & control</subject><subject>Dengue fever</subject><subject>Dengue Virus - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Glycoproteins</subject><subject>Human viral diseases</subject><subject>Immunization</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Neutralization Tests</subject><subject>Pichia - genetics</subject><subject>Proteins</subject><subject>Recombinant Proteins - immunology</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Viral diseases</subject><subject>Viral Envelope Proteins - immunology</subject><subject>Viral Vaccines - immunology</subject><subject>Virology</subject><subject>Viruses</subject><subject>Yeast</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkcuOFCEUhonROO3oA7gxxER3pdyqgKVpr8kkutA1oeBUD5MqaIGaTPtWvqF0upNJZiMLOBy-_wDnR-glJe8oIfJ9aRPpO0J4R-ggO_0IbajgrFNSq8doQzgRnRqIukDPSrkhDSS8f4ouqOilpopv0N-tzWO6O3QV8hKinecDrnmNzlbw-CPE3QpY4NuQ14Ih3sKc9oB388GlfU4VQsRwt89QSsPb5kdw18HivS015VBayq-uHUVYa7Zz-BPiDttYw5h8gNJCj5s6lGqjA1zTwzvddXtTS8Gx-hIcPEdPJjsXeHFeL9Gvz59-br92V9-_fNt-uOqcYKp2DEA5z3vPNQNiJynd5IW0rSV9rxRTigKjgx490aOQExDVMzYR8KDpKBm_RG9Pdds_f69QqllCcTDPNkJai5GUE62F_C9IVRuD4A18_QC8SWtuPS-GUSYIG8TQIHqCXE6lZJjMPofF5oOhxBxdNyfXTTPTHF03umlenQuv4wL-XnG2uQFvzoAtzs5Tbs0O5Z7rmaJKD_wf8Xm4XA</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>MUNE, M</creator><creator>RODRIGUEZ, R</creator><creator>RAMIREZ, R</creator><creator>SOTO, Y</creator><creator>SIERRA, B</creator><creator>RODRIGUEZ ROCHE, R</creator><creator>MARQUEZ, G</creator><creator>GARCIA, J</creator><creator>GUILLEN, G</creator><creator>GUZMAN, M. G</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>Carboxy-terminally truncated Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induced neutralizing antibodies and resistance to Dengue 4 virus challenge in mice</title><author>MUNE, M ; RODRIGUEZ, R ; RAMIREZ, R ; SOTO, Y ; SIERRA, B ; RODRIGUEZ ROCHE, R ; MARQUEZ, G ; GARCIA, J ; GUILLEN, G ; GUZMAN, M. G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-2ee8cd35d392e0af77cfd47a30455882881e2169bd09b47fe08522f0ede91b723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Viral - blood</topic><topic>Biological and medical sciences</topic><topic>Dengue - prevention & control</topic><topic>Dengue fever</topic><topic>Dengue Virus - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genomes</topic><topic>Glycoproteins</topic><topic>Human viral diseases</topic><topic>Immunization</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Neutralization Tests</topic><topic>Pichia - genetics</topic><topic>Proteins</topic><topic>Recombinant Proteins - immunology</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Viral diseases</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viral Vaccines - immunology</topic><topic>Virology</topic><topic>Viruses</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MUNE, M</creatorcontrib><creatorcontrib>RODRIGUEZ, R</creatorcontrib><creatorcontrib>RAMIREZ, R</creatorcontrib><creatorcontrib>SOTO, Y</creatorcontrib><creatorcontrib>SIERRA, B</creatorcontrib><creatorcontrib>RODRIGUEZ ROCHE, R</creatorcontrib><creatorcontrib>MARQUEZ, G</creatorcontrib><creatorcontrib>GARCIA, J</creatorcontrib><creatorcontrib>GUILLEN, G</creatorcontrib><creatorcontrib>GUZMAN, M. G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MUNE, M</au><au>RODRIGUEZ, R</au><au>RAMIREZ, R</au><au>SOTO, Y</au><au>SIERRA, B</au><au>RODRIGUEZ ROCHE, R</au><au>MARQUEZ, G</au><au>GARCIA, J</au><au>GUILLEN, G</au><au>GUZMAN, M. G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carboxy-terminally truncated Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induced neutralizing antibodies and resistance to Dengue 4 virus challenge in mice</atitle><jtitle>Archives of virology</jtitle><addtitle>Arch Virol</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>148</volume><issue>11</issue><spage>2267</spage><epage>2273</epage><pages>2267-2273</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>We have expressed a recombinant Dengue 4 virus envelope glycoprotein (E4rec), truncated at its C-terminus by 53 amino acids, in Pichia pastoris. The presence of E4rec was confirmed by Western-blot using anti-DEN 4 hyper immune mouse ascitic fluid. E4rec migrated during SDS-PAGE as a 64 kDa protein. Treatment with endoglycosidases showed that the E protein was modified by the addition of short mannose chains and the absence of hyperglycosylation. When administered to BALB-C mice, E4rec elicited a DEN 4 neutralizing antibody response haemagglutination inhibition antibodies and specific memory T cell response. Mice immunized were also significantly protected against lethal DEN 4 virus challenge (86.6%, p < 0.001).</abstract><cop>Wien</cop><cop>New York, NY</cop><pub>Springer</pub><pmid>14579183</pmid><doi>10.1007/s00705-003-0167-9</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0304-8608 |
ispartof | Archives of virology, 2003-11, Vol.148 (11), p.2267-2273 |
issn | 0304-8608 1432-8798 |
language | eng |
recordid | cdi_proquest_miscellaneous_71309947 |
source | Springer Nature |
subjects | Amino acids Animals Antibodies Antibodies, Viral - blood Biological and medical sciences Dengue - prevention & control Dengue fever Dengue Virus - immunology Fundamental and applied biological sciences. Psychology Genetic engineering Genomes Glycoproteins Human viral diseases Immunization Infectious diseases Medical sciences Mice Mice, Inbred BALB C Microbiology Miscellaneous Mycology Neutralization Tests Pichia - genetics Proteins Recombinant Proteins - immunology Vaccines, Synthetic - immunology Viral diseases Viral Envelope Proteins - immunology Viral Vaccines - immunology Virology Viruses Yeast |
title | Carboxy-terminally truncated Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induced neutralizing antibodies and resistance to Dengue 4 virus challenge in mice |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T05%3A20%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Carboxy-terminally%20truncated%20Dengue%204%20virus%20envelope%20glycoprotein%20expressed%20in%20Pichia%20pastoris%20induced%20neutralizing%20antibodies%20and%20resistance%20to%20Dengue%204%20virus%20challenge%20in%20mice&rft.jtitle=Archives%20of%20virology&rft.au=MUNE,%20M&rft.date=2003-11-01&rft.volume=148&rft.issue=11&rft.spage=2267&rft.epage=2273&rft.pages=2267-2273&rft.issn=0304-8608&rft.eissn=1432-8798&rft_id=info:doi/10.1007/s00705-003-0167-9&rft_dat=%3Cproquest_cross%3E18888643%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c428t-2ee8cd35d392e0af77cfd47a30455882881e2169bd09b47fe08522f0ede91b723%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=212402646&rft_id=info:pmid/14579183&rfr_iscdi=true |