Differential expression of the nonmuscle-type cofilin gene between subcutaneous and visceral adipose tissue

Visceral adipocytes differ in various biochemical properties from adipocytes of subcutaneous origin. However, information on differences in gene expression between visceral and subcutaneous fat depots is limited. Expression of the genes for the nonmusical and muscle isoforms of the actin-binding pro...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-10, Vol.67 (10), p.2262-2265
Main Authors: Choi, K.C. (Shinshu Univ., Minamiminowa, Nagano (Japan). Faculty of Agriculture), Roh, S.G, Hishikawa, D, Miyahara, H, Kuno, M, Tsuzuki, H, Tomimatsu, A, Hong, Y.H, Cho, K.K, Han, K.H, Sasaki, S
Format: Article
Language:English
Subjects:
Actin Depolymerizing Factors
ADIPOSE TISSUE
Adipose Tissue - metabolism
ANIMAL TISSUES
Animals
BINDING PROTEINS
BIOCHEMISTRY
Biological and medical sciences
Cattle
Diet
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Gene Expression Regulation
Lipid Metabolism
MICE
Microfilament Proteins - genetics
Microfilament Proteins - physiology
MUSCLES
nonmuscle-type cofilin gene
Protein Isoforms - genetics
Protein Isoforms - physiology
RNA
RNA, Messenger - analysis
SKIN
subcutaneous adipocyte
Subcutaneous Tissue
SWINE
Tissue Distribution
Viscera
visceral adipocyte
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Summary:Visceral adipocytes differ in various biochemical properties from adipocytes of subcutaneous origin. However, information on differences in gene expression between visceral and subcutaneous fat depots is limited. Expression of the genes for the nonmusical and muscle isoforms of the actin-binding protein cofilin was examined in subcutaneous and visceral fat depots of mice, pigs, and cattle by semiquantative reverse transcription and polymerase chain reaction analysis. The abundance of nonmuscle-type cofilin mIRNA was markedly higher in visceral adipose tissue than in subcutaneous adipose tissue of mouse and pig. This difference was more pronounced in mice fed a high-fat diet than in those fed a standard diet. In cattle, however, the amount of non-muscle-type cofilin mRNA was greater in subcutaneous fat than in visceral fat. Muscle-type cofilin mRNA was not detected in either adipose tissue of any of the three species. These results suggest that the nonmuscle isoform of cofilin, and therefore the cytoskeleton, may play a role in lipid accumulation in visceral adipose tissue.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.67.2262