Discovering New Insect Viruses: Whitefly Iridovirus (Homoptera: Aleyrodidae: Bemisia tabaci)

Adult whiteflies, Bemisia tabaci (Gennadius), collected from the field were screened for viral pathogens using a cell line from the silverleaf whitefly, B. tabaci, B biotype (syn. B. argentifolii). Homogenates from the field-collected whiteflies were applied to cell cultures and checked for cytopath...

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Bibliographic Details
Published in:Journal of invertebrate pathology 2001-11, Vol.78 (4), p.220-225
Main Authors: Hunter, W.B, Patte, C.P, Sinisterra, X.H, Achor, D.S, Funk, C.J, Polston, J.E
Format: Article
Language:English
Subjects:
Aleyrodidae
Animals
Bemisia
Bemisia tabaci
Biological and medical sciences
Biological control
biological insecticide
Cell Line
Control
DNA, Viral - analysis
Fundamental and applied biological sciences. Psychology
Hemiptera
Hemiptera - virology
Homoptera
Invertebrate iridescent virus 6
Iridovirus
Iridovirus - classification
Iridovirus - genetics
Iridovirus - isolation & purification
lacewings
leafhoppers
Microbiology
pathogen
Phytopathology. Animal pests. Plant and forest protection
Protozoa. Invertebrates
Techniques used in virology
Virology
virus
whitefly
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Summary:Adult whiteflies, Bemisia tabaci (Gennadius), collected from the field were screened for viral pathogens using a cell line from the silverleaf whitefly, B. tabaci, B biotype (syn. B. argentifolii). Homogenates from the field-collected whiteflies were applied to cell cultures and checked for cytopathic effects (CPE). Cells were observed to develop cytoplasmic inclusions and to have a change in morphology. Cells displaying CPE were observed using a transmission electron microscope and found to be infected with a virus. The virus particles had an icosahedral shape and an approximate size of 120–130 nm. The virus was observed in defined areas of the cytoplasm adjacent to the cell nucleus. Analysis using polymerase chain reaction, Southern blot hybridization, and DNA sequencing confirmed that the virus discovered infecting the whitefly cell cultures was an iridovirus. Sequence analysis showed that the amplimer (893 bp) had a 95% homology to the invertebrate iridescent virus type 6 major capsid protein gene. Discovery of new viruses of whiteflies may provide renewed interest in using pathogens in the development of innovative management strategies. This is the first report of an iridescent virus isolated from whiteflies, B. tabaci, collected from the field.
ISSN:0022-2011
1096-0805
DOI:10.1006/jipa.2001.5060