Fine Oral Filaments in Paramecium: a Biochemical and Immunological Analysis

• Published in: The Journal of eukaryotic microbiology 2001-03, Vol.48 (2), p.234-245
• Main Authors: CLEROT, JEAN-CLAUDE, IFTODE, FRANCINE, BUDIN, KARINE, JEANMAIRE-WOLF, RACHEL, COFFE, GÉRARD, FLEURY-AUBUSSON, ANNE
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Protozoan - immunology; Antigens, Protozoan - analysis; Biological and medical sciences; Cell fractionation; cell shaping; ciliates; cytoskeletal assembly; Cytoskeletal Proteins - immunology; Epitopes; Fundamental and applied biological sciences. Psychology; Immunoblotting; immunofluorescence; immunogold labeling; Immunohistochemistry; Interphase; Microscopy, Fluorescence; Microscopy, Immunoelectron; monoclonal antibodies; Morphogenesis; Paramecium - chemistry; Paramecium - growth & development; Paramecium - immunology; Paramecium - ultrastructure; Protozoa; Protozoan Proteins - analysis; Protozoan Proteins - immunology; Systematics. Geographical distribution. Morphology. Cytology; tetrins; ultrastructure of filamentous networks
• ISSN: 1066-5234; 1550-7408
• DOI: 10.1111/j.1550-7408.2001.tb00310.x

In Paramecium, several kinds of the oral networks of fine filaments are defined at the ultrastructural level. Using the sodium chloride-treated oral apparatus of Paramecium as an antigen to produce monoclonal antibodies, we have begun to identify the proteins constituting these networks. Immunoblotting showed that all positive antibodies were directed against three bands (70-, 75- and 83-kD), which corresponded to quantitatively minor components of the antigen; there was no antibody specific for the quantitatively major components (58- and 62-kD). Immunolocalization with four of these antibodies directed against one or several of these three bands showed that these proteins are components of the fine filaments supporting the oral area; a decoration of the basal bodies and the outer lattice was also observed on the cortex. Immunofluorescence on interphase cells suggested that the three proteins colocalized on the left side of the oral apparatus, whereas only the 70-kD band was detected on the right side. During division, the antigens of the antibodies were detected at different stages after oral basal body assembly. The antibodies cross-reacted with the tetrins, which are oral filament-forming proteins in Tetrahymena, demonstrating that tetrin-related proteins are quantitatively minor components of the oral and the somatic cytoskeleton of Paramecium.