Allergen-specific IgG and IgA in serum and bronchoalveolar lavage fluid in a model of experimental feline asthma

Allergic asthma, a Th2 cell driven response to inhaled allergens, has classically been thought of as predominantly mediated by IgE antibodies. To investigate the role of other immunoglobulin classes (e.g., IgG and IgA) in the immunopathogenesis of allergic asthma, levels of these allergen-specific i...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2003-12, Vol.96 (3), p.119-127
Main Authors: Norris, C.R, Byerly, J.R, Decile, K.C, Berghaus, R.D, Walby, W.F, Schelegle, E.S, Hyde, D.M, Gershwin, L.J
Format: Article
Language:English
Subjects:
Allergens - immunology
Allergy
Animals
Antibody
Asthma - immunology
Asthma - veterinary
Bronchial Hyperreactivity
Bronchial Provocation Tests - veterinary
Bronchoalveolar Lavage Fluid - immunology
Cat
Cat Diseases - immunology
Cats
Cynodon - immunology
Cynodon dactylon
ELISA
Enzyme-Linked Immunosorbent Assay - veterinary
Immunoglobulin
Immunoglobulin A - blood
Immunoglobulin A - immunology
Immunoglobulin G - blood
Immunoglobulin G - immunology
Methacholine Chloride - immunology
Pulmonary
Respiratory Function Tests - veterinary
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Summary:Allergic asthma, a Th2 cell driven response to inhaled allergens, has classically been thought of as predominantly mediated by IgE antibodies. To investigate the role of other immunoglobulin classes (e.g., IgG and IgA) in the immunopathogenesis of allergic asthma, levels of these allergen-specific immunoglobulins were measured in serum and mucosal fluids. Bermuda grass allergen (BGA)-specific IgG and IgA ELISAs in serum and bronchoalveolar lavage fluid (BALF) were developed and optimized in an experimental model of BGA-induced feline asthma. Levels of BGA-specific IgG and IgA significantly increased over time in serum and BALF after allergen sensitization. Additionally, these elevated levels of BGA-specific IgG and IgA were seen in conjunction with the development of an asthmatic phenotype indicated by positive intradermal skin tests, enhanced airways hyperreactivity, and increased eosinophil percentages in the BALF.
ISSN:0165-2427
1873-2534
DOI:10.1016/S0165-2427(03)00144-2