Proton HR-MAS spectroscopy and quantitative pathologic analysis of MRI/3D-MRSI-targeted postsurgical prostate tissues

• Published in: Magnetic resonance in medicine 2003-11, Vol.50 (5), p.944-954
• Main Authors: Swanson, Mark G., Vigneron, Daniel B., Tabatabai, Z. Laura, Males, Ryan G., Schmitt, Lars, Carroll, Peter R., James, Joyce K., Hurd, Ralph E., Kurhanewicz, John
• Format: Article
• Language: English
• Subjects: 1H high-resolution magic angle spinning (1H HR-MAS); Aged; and polyamine metabolism; Biological and medical sciences; Biomarkers, Tumor - analysis; choline; choline-containing compounds; citrate; citrate, choline, and polyamine metabolism; glycerophosphocholine; Humans; Imaging, Three-Dimensional; Magnetic Resonance Imaging - methods; magnetic resonance spectroscopic imaging (MRSI); Magnetic Resonance Spectroscopy - methods; Male; Medical sciences; Middle Aged; phosphocholine; Prostate - metabolism; Prostate - pathology; prostate cancer; Prostatectomy; Protons; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Technology. Biomaterials. Equipments. Material. Instrumentation
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/mrm.10614

Proton high‐resolution magic angle spinning (1H HR‐MAS) NMR spectroscopy and quantitative histopathology were performed on the same 54 MRI/3D‐MRSI‐targeted postsurgical prostate tissue samples. Presurgical MRI/3D‐MRSI targeted healthy and malignant prostate tissues with an accuracy of 81%. Even in the presence of substantial tissue heterogeneity, distinct 1H HR‐MAS spectral patterns were observed for different benign tissue types and prostate cancer. Specifically, healthy glandular tissue was discriminated from prostate cancer based on significantly higher levels of citrate (P = 0.04) and polyamines (P = 0.01), and lower (P = 0.02) levels of the choline‐containing compounds choline, phosphocholine (PC), and glycerophosphocholine (GPC). Predominantly stromal tissue lacked both citrate and polyamines, but demonstrated significantly (P = 0.01) lower levels of choline compounds than cancer. In addition, taurine, myo‐inositol, and scyllo‐inositol were all higher in prostate cancer vs. healthy glandular and stromal tissues. Among cancer samples, larger increases in choline, and decreases in citrate and polyamines (P = 0.05) were observed with more aggressive cancers, and a MIB‐1 labeling index correlated (r = 0.62, P = 0.01) with elevated choline. The elucidation of spectral patterns associated with mixtures of different prostate tissue types and cancer grades, and the inclusion of new metabolic markers for prostate cancer may significantly improve the clinical interpretation of in vivo prostate MRSI data. Magn Reson Med 50:944–954, 2003. © 2003 Wiley‐Liss, Inc.