Observation of Antigen-Dependent CD8+ T-Cell/ Dendritic Cell Interactions in Vivo

In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tiss...

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Bibliographic Details
Published in:Cellular immunology 2001-12, Vol.214 (2), p.110-122
Main Authors: Schaefer, Brian C., Schaefer, Michele L., Kappler, John W., Marrack, Philippa, Kedl, Ross M.
Format: Article
Language:English
Subjects:
Adoptive Transfer
Animals
Antigen Presentation
Antigens - immunology
CD8+ T cell
CD8-Positive T-Lymphocytes - immunology
Cell Communication
Cell Lineage
Cell Movement
Chemotaxis, Leukocyte
dendritic cell
Dendritic Cells - immunology
flow cytometry
Fluorescent Dyes - analysis
Gene Expression Regulation
Genes, Reporter
green fluorescent protein
Green Fluorescent Proteins
histology
immunofluorescence microscopy
Luminescent Proteins - analysis
Lymph Nodes - cytology
Lymph Nodes - immunology
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Organ Specificity
Promoter Regions, Genetic
Radiation Chimera
Recombinant Fusion Proteins - analysis
Spleen - cytology
Spleen - immunology
transgenic mouse
UbC promoter
Ubiquitin C - genetics
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Summary:In order to track hematopoetic cells of all lineages unambiguously at all stages of development, we have developed C57BL/6 mice that express a transgene coding for green fluorescent protein (GFP) under control of the human ubiquitin C promoter. These mice, called UBI-GFP/BL6, express GFP in all tissues examined, with high levels of GFP expression observed in hematopoetic cells. UBI-GFP/BL6 mice are unique in that B cells, T cells, and dendritic cells have distinct levels of GFP fluorescence. In cell transfer experiments, leukocytes from UBI-GFP/BL6 mice are readily identified by FACS or fluorescence microscopy. We demonstrate that transplanted UBI-GFP/BL6 dendritic cells are easily identified in secondary lymphoid tissues. Direct interactions between individual dendritic cells and multiple naı̈ve CD8+ T cells are observed in lymph nodes within 12 h of cell transfer and require loading of the dendritic cells with the appropriate peptide antigen. Dendritic cells undergo specific morphologic changes following interactions with antigen-specific T cells.
ISSN:0008-8749
1090-2163
DOI:10.1006/cimm.2001.1895