Generation of bioreagents for protein chips

• Published in: Proteomics (Weinheim) 2003-11, Vol.3 (11), p.2123-2134
• Main Authors: Phelan, Michael L., Nock, Steffen
• Format: Article
• Language: English
• Subjects: Affinity Labels - chemistry; Animals; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - immunology; Antigens - chemistry; Antigens - immunology; Capture agents; Fluorescent Dyes - chemistry; Humans; Polymers - chemistry; Protein Array Analysis; Protein chips; Protein Conformation; Recombinant Proteins - chemistry; Recombinant Proteins - immunology; Review
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.200300596

Protein microarrays have the potential to dramatically increase the throughput of proteomic analysis. Protein expression profiling chips with distinct spots of immobilized protein capture agents will allow the simultaneous measurement of hundreds to thousands of proteins from one sample. In contrast to DNA chips, for which the capture probes are easily designed and synthesized, the development of content for protein biochips is a long and laborious process. Careful consideration must be given to the specificities desired, the format of the assay, and the requirements of the capture agents, as well as to process optimization to minimize development time and cost. Monoclonal antibodies have been the prime choice as protein capture agents for the majority of protein chips developed to date. New technologies for the production of protein capture agents are more amenable to automation than traditional monoclonal antibody production and therefore carry the promise for industrialization.