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Pea lectin expressed transgenically in oilseed rape reduces growth rate of pollen beetle larvae
In several studies plant lectins have shown promise as transgenic resistance factors against various insect pests. We have here shown that pea seed lectin is a potential candidate for use against pollen beetle, a serious pest of Brassica oilseeds. In feeding assays where pollen beetle larvae were fe...
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Published in: | Transgenic research 2003-10, Vol.12 (5), p.555-567 |
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description | In several studies plant lectins have shown promise as transgenic resistance factors against various insect pests. We have here shown that pea seed lectin is a potential candidate for use against pollen beetle, a serious pest of Brassica oilseeds. In feeding assays where pollen beetle larvae were fed oilseed rape anthers soaked in a 1% solution of pea lectin there was a reduction in survival of 84% compared to larvae on control treatment and the weight of surviving larvae was reduced by 79%. When a 10% solution of pea lectin was used all larvae were dead after 4 days of testing. To further evaluate the potential use of pea lectin, transgenic plants of oilseed rape (Brassica napus cv. Westar) were produced in which the pea lectin gene under control of the pollen-specific promoter Sta44-4 was introduced. In 11 out of 20 tested plants of the T0-generation there was a significant reduction in larval weight, which ranged up to 46% compared to the control. A small but significant reduction in larval survival rate was also observed. In the T2-generation significant weight reductions, with a maximum of 32%, were obtained in 10 out of 33 comparisons between transgenic plants and their controls. Pea lectin concentrations in anthers of transgenic T2-plants ranged up to 1.5% of total soluble protein. There was a negative correlation between lectin concentration and larval growth. Plants from test groups with significant differences in larval weights had a significantly higher mean pea lectin concentration, 0.64% compared to 0.15% for plants from test groups without effect on larval weight. These results support the conclusion that pea lectin is a promising resistance factor for use in Brassica oilseeds against pollen beetles. |
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We have here shown that pea seed lectin is a potential candidate for use against pollen beetle, a serious pest of Brassica oilseeds. In feeding assays where pollen beetle larvae were fed oilseed rape anthers soaked in a 1% solution of pea lectin there was a reduction in survival of 84% compared to larvae on control treatment and the weight of surviving larvae was reduced by 79%. When a 10% solution of pea lectin was used all larvae were dead after 4 days of testing. To further evaluate the potential use of pea lectin, transgenic plants of oilseed rape (Brassica napus cv. Westar) were produced in which the pea lectin gene under control of the pollen-specific promoter Sta44-4 was introduced. In 11 out of 20 tested plants of the T0-generation there was a significant reduction in larval weight, which ranged up to 46% compared to the control. A small but significant reduction in larval survival rate was also observed. In the T2-generation significant weight reductions, with a maximum of 32%, were obtained in 10 out of 33 comparisons between transgenic plants and their controls. Pea lectin concentrations in anthers of transgenic T2-plants ranged up to 1.5% of total soluble protein. There was a negative correlation between lectin concentration and larval growth. Plants from test groups with significant differences in larval weights had a significantly higher mean pea lectin concentration, 0.64% compared to 0.15% for plants from test groups without effect on larval weight. These results support the conclusion that pea lectin is a promising resistance factor for use in Brassica oilseeds against pollen beetles.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1023/A:1025813526283</identifier><identifier>PMID: 14601654</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Animals ; Biological and medical sciences ; Biotechnology ; Blotting, Western ; Brassica ; Brassica napus - genetics ; Brassica napus - metabolism ; Coleoptera - genetics ; Coleoptera - growth & development ; Concanavalin A - genetics ; Concanavalin A - metabolism ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Larva - growth & development ; Meligethes ; Methods. Procedures. Technologies ; Pisum sativum ; Pisum sativum - genetics ; Plant Lectins - genetics ; Plant Lectins - metabolism ; Plants, Genetically Modified ; Rape plants ; Transgenic animals and transgenic plants</subject><ispartof>Transgenic research, 2003-10, Vol.12 (5), p.555-567</ispartof><rights>2004 INIST-CNRS</rights><rights>Kluwer Academic Publishers 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-284226c00e7c5035a1ef2a989ba154199e91c204e0e42270866be3036127a6f53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15206011$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14601654$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MELANDER, Margareta</creatorcontrib><creatorcontrib>AHMAN, Inger</creatorcontrib><creatorcontrib>KAMNERT, Iréne</creatorcontrib><creatorcontrib>STRÖMDAHL, Ann-Charlotte</creatorcontrib><title>Pea lectin expressed transgenically in oilseed rape reduces growth rate of pollen beetle larvae</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><description>In several studies plant lectins have shown promise as transgenic resistance factors against various insect pests. We have here shown that pea seed lectin is a potential candidate for use against pollen beetle, a serious pest of Brassica oilseeds. In feeding assays where pollen beetle larvae were fed oilseed rape anthers soaked in a 1% solution of pea lectin there was a reduction in survival of 84% compared to larvae on control treatment and the weight of surviving larvae was reduced by 79%. When a 10% solution of pea lectin was used all larvae were dead after 4 days of testing. To further evaluate the potential use of pea lectin, transgenic plants of oilseed rape (Brassica napus cv. Westar) were produced in which the pea lectin gene under control of the pollen-specific promoter Sta44-4 was introduced. In 11 out of 20 tested plants of the T0-generation there was a significant reduction in larval weight, which ranged up to 46% compared to the control. A small but significant reduction in larval survival rate was also observed. In the T2-generation significant weight reductions, with a maximum of 32%, were obtained in 10 out of 33 comparisons between transgenic plants and their controls. Pea lectin concentrations in anthers of transgenic T2-plants ranged up to 1.5% of total soluble protein. There was a negative correlation between lectin concentration and larval growth. Plants from test groups with significant differences in larval weights had a significantly higher mean pea lectin concentration, 0.64% compared to 0.15% for plants from test groups without effect on larval weight. These results support the conclusion that pea lectin is a promising resistance factor for use in Brassica oilseeds against pollen beetles.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Western</subject><subject>Brassica</subject><subject>Brassica napus - genetics</subject><subject>Brassica napus - metabolism</subject><subject>Coleoptera - genetics</subject><subject>Coleoptera - growth & development</subject><subject>Concanavalin A - genetics</subject><subject>Concanavalin A - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Larva - growth & development</subject><subject>Meligethes</subject><subject>Methods. Procedures. Technologies</subject><subject>Pisum sativum</subject><subject>Pisum sativum - genetics</subject><subject>Plant Lectins - genetics</subject><subject>Plant Lectins - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Rape plants</subject><subject>Transgenic animals and transgenic plants</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqF0M9L7DAQB_Agiu5Tz94kCL5bdSZp0sTbIuoTBD3ouWSzU61k25q0_vjvDbgieHmngZlPhsmXsQOEEwQhT-dnuSiDUgktjNxgM1SVLKzUZpPNwGpRGIN2h_1J6RkAAYzcZjtYakCtyhmr78jxQH5sO07vQ6SUaMnH6Lr0SF3rXQgfPM_6NiTKk-gG4pGWk6fEH2P_Nj7l3ki8b_jQh0AdXxCNgXhw8dXRHttqXH66v6677OHy4v78X3Fze3V9Pr8pvCxxLIQphdAegCqvQCqH1AhnjV04VCVaSxa9gJKAMqzAaL0gCVKjqJxulNxlf7_2DrF_mSiN9apNnkJwHfVTqiuUsrTl_yFaISHnmeHRL_jcT7HLn6grJQDymTqjwzWaFita1kNsVy5-1N8BZ3C8Bi7lMJscrG_Tj8ubskT5CZdIh-I</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>MELANDER, Margareta</creator><creator>AHMAN, Inger</creator><creator>KAMNERT, Iréne</creator><creator>STRÖMDAHL, Ann-Charlotte</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20031001</creationdate><title>Pea lectin expressed transgenically in oilseed rape reduces growth rate of pollen beetle larvae</title><author>MELANDER, Margareta ; AHMAN, Inger ; KAMNERT, Iréne ; STRÖMDAHL, Ann-Charlotte</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-284226c00e7c5035a1ef2a989ba154199e91c204e0e42270866be3036127a6f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Western</topic><topic>Brassica</topic><topic>Brassica napus - genetics</topic><topic>Brassica napus - metabolism</topic><topic>Coleoptera - genetics</topic><topic>Coleoptera - growth & development</topic><topic>Concanavalin A - genetics</topic><topic>Concanavalin A - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Larva - growth & development</topic><topic>Meligethes</topic><topic>Methods. Procedures. Technologies</topic><topic>Pisum sativum</topic><topic>Pisum sativum - genetics</topic><topic>Plant Lectins - genetics</topic><topic>Plant Lectins - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Rape plants</topic><topic>Transgenic animals and transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MELANDER, Margareta</creatorcontrib><creatorcontrib>AHMAN, Inger</creatorcontrib><creatorcontrib>KAMNERT, Iréne</creatorcontrib><creatorcontrib>STRÖMDAHL, Ann-Charlotte</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Databases</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MELANDER, Margareta</au><au>AHMAN, Inger</au><au>KAMNERT, Iréne</au><au>STRÖMDAHL, Ann-Charlotte</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pea lectin expressed transgenically in oilseed rape reduces growth rate of pollen beetle larvae</atitle><jtitle>Transgenic research</jtitle><addtitle>Transgenic Res</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>12</volume><issue>5</issue><spage>555</spage><epage>567</epage><pages>555-567</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>In several studies plant lectins have shown promise as transgenic resistance factors against various insect pests. We have here shown that pea seed lectin is a potential candidate for use against pollen beetle, a serious pest of Brassica oilseeds. In feeding assays where pollen beetle larvae were fed oilseed rape anthers soaked in a 1% solution of pea lectin there was a reduction in survival of 84% compared to larvae on control treatment and the weight of surviving larvae was reduced by 79%. When a 10% solution of pea lectin was used all larvae were dead after 4 days of testing. To further evaluate the potential use of pea lectin, transgenic plants of oilseed rape (Brassica napus cv. Westar) were produced in which the pea lectin gene under control of the pollen-specific promoter Sta44-4 was introduced. In 11 out of 20 tested plants of the T0-generation there was a significant reduction in larval weight, which ranged up to 46% compared to the control. A small but significant reduction in larval survival rate was also observed. In the T2-generation significant weight reductions, with a maximum of 32%, were obtained in 10 out of 33 comparisons between transgenic plants and their controls. Pea lectin concentrations in anthers of transgenic T2-plants ranged up to 1.5% of total soluble protein. There was a negative correlation between lectin concentration and larval growth. Plants from test groups with significant differences in larval weights had a significantly higher mean pea lectin concentration, 0.64% compared to 0.15% for plants from test groups without effect on larval weight. These results support the conclusion that pea lectin is a promising resistance factor for use in Brassica oilseeds against pollen beetles.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>14601654</pmid><doi>10.1023/A:1025813526283</doi><tpages>13</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Biotechnology Blotting, Western Brassica Brassica napus - genetics Brassica napus - metabolism Coleoptera - genetics Coleoptera - growth & development Concanavalin A - genetics Concanavalin A - metabolism Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Larva - growth & development Meligethes Methods. Procedures. Technologies Pisum sativum Pisum sativum - genetics Plant Lectins - genetics Plant Lectins - metabolism Plants, Genetically Modified Rape plants Transgenic animals and transgenic plants |
title | Pea lectin expressed transgenically in oilseed rape reduces growth rate of pollen beetle larvae |
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