Solution structure and stability of a disulfide cross-linked nucleopeptide duplex

NMR methods are used to study the structure and stability of the duplex formed by the nucleopeptide [Ac-Cys-Gly-Ala-Hse(p3'dGCATGC)-Ala-OH]2[S-S], in which the oligonucleotide is self-complementary and the cysteine residues of the two peptide chains form a disulfide bridge; thermal transitions...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2003-01 (20), p.2558-2559
Main Authors: Gómez-Pinto, Irene, Marchán, Vicente, Gago, Federico, Grandas, Anna, González, Carlos
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Cysteine - chemistry
Disulfides - chemistry
Models, Molecular
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
Nucleic Acid Conformation
Nucleic Acid Denaturation
Nucleoproteins - chemistry
Oligonucleotides - chemistry
Protein Conformation
Solutions - chemistry
Temperature
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Summary:NMR methods are used to study the structure and stability of the duplex formed by the nucleopeptide [Ac-Cys-Gly-Ala-Hse(p3'dGCATGC)-Ala-OH]2[S-S], in which the oligonucleotide is self-complementary and the cysteine residues of the two peptide chains form a disulfide bridge; thermal transitions and NMR-derived structural calculations are consistent with a 3-D structure in which the oligonucleotide forms a standard B-DNA helix without significant distortions; the peptide chains are relatively disordered in solution and lie in the minor groove of the DNA helix; this nucleopeptide duplex exhibits a high melting temperature, indicating that peptide-oligonucleotide conjugates containing cysteines are suitable molecules to establish cross-links between DNA strands and stabilize the duplex.
ISSN:1359-7345
1364-548X
DOI:10.1039/b307300a