PM19, a barley (Hordeum vulgare L.) gene encoding a putative plasma membrane protein, is expressed during embryo development and dormancy

• Published in: Journal of experimental botany 2002-01, Vol.53 (366), p.147-148
• Main Authors: Ranford, Julia C., Bryce, James H., Morris, Peter C.
• Format: Article
• Language: English
• Subjects: ABA; Agronomy. Soil science and plant productions; Amino Acid Sequence; Barley; Biological and medical sciences; Cell Membrane - genetics; Cell Membrane - metabolism; Cell membranes; Classical and quantitative genetics. Population genetics. Molecular genetics; Complementary DNA; Dormancy; Economic plant physiology; embryo; Embryo development. Germination; Embryogenesis; Embryos; Freezing; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental - drug effects; Gene Expression Regulation, Plant - drug effects; GENE NOTE; Generalities. Genetics. Plant material; Genes; Genetics and breeding of economic plants; Germination; Germination - drug effects; Germination - genetics; Germination and dormancy; Growth and development; Hordeum - genetics; Hordeum - metabolism; Hordeum vulgare; Membrane Proteins - genetics; Membrane Proteins - metabolism; Molecular genetics; Molecular Sequence Data; Oats; Plant physiology and development; Plant Proteins - genetics; Plant Proteins - metabolism; PM19 gene; RNA, Messenger - drug effects; RNA, Messenger - genetics; RNA, Messenger - metabolism; Seeds - genetics; Seeds - metabolism; Sequence Homology, Amino Acid; Sodium Chloride - pharmacology; sorbitol; Sorbitol - pharmacology; stress
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jexbot/53.366.147

A barley (Hordeum vulgare L.) cDNA, PM19, encoding a putative plasma membrane protein was isolated through differential screening of a dormant wild oat embryo library. PM19 is expressed in barley embryos from mid‐embryogenesis up to maturity. PM19 mRNA levels decline upon germination, whereas dormant embryos retained high levels of message for up to 72 h of imbibition. PM19 mRNA levels also remained high or were reinduced in non‐dormant embryos by treatments that prevented germination (250 mm NaCl, 10% sorbitol, or 50 μm ABA). The PM19 protein sequence is highly conserved in monocotyledonous and dicotyledonous plants.