Oral vaccination with Mycobacterium bovis BCG in a lipid formulation induces resistance to pulmonary tuberculosis in brushtail possums

A method was developed for formulating Mycobacterium bovis bacille Calmette–Guerin (BCG) for oral vaccination against tuberculosis. Selected lipid-based formulations of BCG were tested in the brushtail possum for their ability to elicit immune responses and protection against bovine tuberculosis. Fo...

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Bibliographic Details
Published in:Vaccine 2003-12, Vol.22 (1), p.70-76
Main Authors: Aldwell, Frank E, Keen, Denise L, Parlane, Natalie A, Skinner, Margot A, de Lisle, Geoffrey W, Buddle, Bryce M
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Administration, Oral
Aerosols
Animals
Applied microbiology
BCG
BCG Vaccine - administration & dosage
BCG Vaccine - immunology
Biological and medical sciences
Chemistry, Pharmaceutical
Fundamental and applied biological sciences. Psychology
Lipids
Lung - microbiology
Lung - pathology
Lymphocyte Count
Microbiology
Mycobacterium bovis
Mycobacterium tuberculosis
Opossums - immunology
Time Factors
Tuberculosis
Tuberculosis, Pulmonary - immunology
Tuberculosis, Pulmonary - microbiology
Tuberculosis, Pulmonary - prevention & control
Vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Summary:A method was developed for formulating Mycobacterium bovis bacille Calmette–Guerin (BCG) for oral vaccination against tuberculosis. Selected lipid-based formulations of BCG were tested in the brushtail possum for their ability to elicit immune responses and protection against bovine tuberculosis. Formulation of BCG in lipid matrices maintained bacteria in a dormant but viable state. Oral delivery of 2×10 8 colony forming units of formulated BCG to possums induced strong lymphocyte proliferation responses to bovine purified protein derivative (PPD) in peripheral blood lymphocytes. Oral vaccination of possums also reduced the severity of disease following aerosol challenge with virulent M. bovis compared with animals vaccinated with non-formulated BCG. In a second experiment, levels of protection with lipid-formulated oral BCG were similar to those seen with subcutaneous BCG vaccination. Our data shows that formulated oral BCG is an efficient means of inducing protection against bovine tuberculosis in possums and should be a practical means of vaccinating wildlife against tuberculosis.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(03)00539-5