Relationship between endothelin 1 and nitric oxide system in the corpus luteum regression

The present study was designed to investigate the relationship between the nitric oxide (NO) system and endothelin 1 (ET-1) in the mechanism of corpus luteum (CL) development and consequently regression in rats. We first evaluated basal ET-1 levels in ovarian tissue from rats with different stages o...

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Bibliographic Details
Published in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2003-11, Vol.69 (5), p.359-364
Main Authors: Tognetti, T., Estevez, A., Luchetti, C.G., Sander, V., Franchi, A.M., Motta, A.B.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Corpus luteum
Culture Techniques
Dinoprost - metabolism
Endothelin-1
Endothelin-1 - metabolism
Enzyme Inhibitors - metabolism
Female
Fundamental and applied biological sciences. Psychology
Humans
Isoenzymes - metabolism
Luteolysis - physiology
NG-Nitroarginine Methyl Ester - metabolism
Nitric Oxide - metabolism
Nitric oxide synthase
Nitric Oxide Synthase - metabolism
Ovary - anatomy & histology
Ovary - metabolism
Progesterone
Progesterone - metabolism
Prostaglandin F2α
Pseudopregnancy
Rats
Vertebrates: endocrinology
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Summary:The present study was designed to investigate the relationship between the nitric oxide (NO) system and endothelin 1 (ET-1) in the mechanism of corpus luteum (CL) development and consequently regression in rats. We first evaluated basal ET-1 levels in ovarian tissue from rats with different stages of CL development. An increased ovarian ET-1 content was found during CL regression. In a dose-department response, ET-1 decreased progesterone (P4) and increased prostaglandin (PG) PGF2 α production. By means of a competitive nitric oxide synthase (NOS) inhibitor: L-nitro arginine methyl ester (L-NAME) and a slow NO releasing: diethyl-aminetriamine (DETA-NONOate), we demonstrated that NO system could be the intermediary in the ET-1 diminishing P4 production. The Western blot analysis revealed an increase on iNOS while eNOS protein expression was diminished. We also found a diminution of total NOS activity after ET-1 treatment. These data suggest the existence of a functional relationship between ET-1 and NOS isoforms leading the regulation of CL functionally.
ISSN:0952-3278
1532-2823
DOI:10.1016/j.plefa.2003.07.002