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Differential Gene Expression of Organic Anion Transporters in Male and Female Rats
Sex-related differential gene expression of organic anion transporters (rOAT1, rOAT2, and rOAT3) in rat brain, liver, and kidney was investigated. There were no sex differences in the expression of rOAT1 mRNA. rOAT2 mRNA was abundant in the liver and weakly expressed in the kidney of male rats; howe...
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| Published in: | Biochemical and biophysical research communications 2002-01, Vol.290 (1), p.482-487 |
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| cited_by | cdi_FETCH-LOGICAL-c406t-9af57c9868a94e8d5b596263a0582abba4322ebb3f5a92717625e4dcdeaefed53 |
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| cites | cdi_FETCH-LOGICAL-c406t-9af57c9868a94e8d5b596263a0582abba4322ebb3f5a92717625e4dcdeaefed53 |
| container_end_page | 487 |
| container_issue | 1 |
| container_start_page | 482 |
| container_title | Biochemical and biophysical research communications |
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| creator | Kobayashi, Yasuna Hirokawa, Noriko Ohshiro, Naomi Sekine, Takashi Sasaki, Tadanori Tokuyama, Shogo Endou, Hitoshi Yamamoto, Toshinori |
| description | Sex-related differential gene expression of organic anion transporters (rOAT1, rOAT2, and rOAT3) in rat brain, liver, and kidney was investigated. There were no sex differences in the expression of rOAT1 mRNA. rOAT2 mRNA was abundant in the liver and weakly expressed in the kidney of male rats; however, the OAT2 gene was strongly expressed in both organs of females. The abundance of rOAT2 mRNA markedly increased in castrated male rat kidney; however, treatment of castrated male rats with testosterone led to a decrease of rOAT2 mRNA. Expression of rOAT3 mRNA in intact female rats was found in the kidney and brain, whereas in males rOAT3 mRNA was also found in the liver. rOAT3 mRNA markedly decreased in the liver of castrated male rats but increased in testosterone-treated castrated male rats. Moreover, rOAT3 mRNA increased in the hypophysectomized female rat liver, indicating that rOAT3 is an inducible isoform. The present findings suggest that sex steroids play an important role in the expression and maintenance of OAT2/3 isoforms in the rat liver and kidney. Our results provide information on the differential gene expression of OAT isoforms with sex hormone dependency. |
| doi_str_mv | 10.1006/bbrc.2001.6180 |
| format | article |
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There were no sex differences in the expression of rOAT1 mRNA. rOAT2 mRNA was abundant in the liver and weakly expressed in the kidney of male rats; however, the OAT2 gene was strongly expressed in both organs of females. The abundance of rOAT2 mRNA markedly increased in castrated male rat kidney; however, treatment of castrated male rats with testosterone led to a decrease of rOAT2 mRNA. Expression of rOAT3 mRNA in intact female rats was found in the kidney and brain, whereas in males rOAT3 mRNA was also found in the liver. rOAT3 mRNA markedly decreased in the liver of castrated male rats but increased in testosterone-treated castrated male rats. Moreover, rOAT3 mRNA increased in the hypophysectomized female rat liver, indicating that rOAT3 is an inducible isoform. The present findings suggest that sex steroids play an important role in the expression and maintenance of OAT2/3 isoforms in the rat liver and kidney. Our results provide information on the differential gene expression of OAT isoforms with sex hormone dependency.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.2001.6180</identifier><identifier>PMID: 11779196</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blotting, Northern ; Brain - metabolism ; Female ; Gene Expression Regulation ; Kidney - metabolism ; Liver - metabolism ; Male ; organic anion ; Organic Anion Transport Protein 1 - biosynthesis ; Organic Anion Transport Protein 1 - genetics ; organic anion transporter ; Organic Anion Transporters - biosynthesis ; Organic Anion Transporters - genetics ; Organic Anion Transporters, Sodium-Independent - biosynthesis ; Organic Anion Transporters, Sodium-Independent - genetics ; pharmacokinetics ; Protein Isoforms ; Rats ; Rats, Wistar ; RNA - metabolism ; RNA, Messenger - metabolism ; sex differences ; Sex Factors ; Tissue Distribution</subject><ispartof>Biochemical and biophysical research communications, 2002-01, Vol.290 (1), p.482-487</ispartof><rights>2002 Elsevier Science (USA)</rights><rights>(c)2002 Elsevier Science.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-9af57c9868a94e8d5b596263a0582abba4322ebb3f5a92717625e4dcdeaefed53</citedby><cites>FETCH-LOGICAL-c406t-9af57c9868a94e8d5b596263a0582abba4322ebb3f5a92717625e4dcdeaefed53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11779196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kobayashi, Yasuna</creatorcontrib><creatorcontrib>Hirokawa, Noriko</creatorcontrib><creatorcontrib>Ohshiro, Naomi</creatorcontrib><creatorcontrib>Sekine, Takashi</creatorcontrib><creatorcontrib>Sasaki, Tadanori</creatorcontrib><creatorcontrib>Tokuyama, Shogo</creatorcontrib><creatorcontrib>Endou, Hitoshi</creatorcontrib><creatorcontrib>Yamamoto, Toshinori</creatorcontrib><title>Differential Gene Expression of Organic Anion Transporters in Male and Female Rats</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Sex-related differential gene expression of organic anion transporters (rOAT1, rOAT2, and rOAT3) in rat brain, liver, and kidney was investigated. There were no sex differences in the expression of rOAT1 mRNA. rOAT2 mRNA was abundant in the liver and weakly expressed in the kidney of male rats; however, the OAT2 gene was strongly expressed in both organs of females. The abundance of rOAT2 mRNA markedly increased in castrated male rat kidney; however, treatment of castrated male rats with testosterone led to a decrease of rOAT2 mRNA. Expression of rOAT3 mRNA in intact female rats was found in the kidney and brain, whereas in males rOAT3 mRNA was also found in the liver. rOAT3 mRNA markedly decreased in the liver of castrated male rats but increased in testosterone-treated castrated male rats. Moreover, rOAT3 mRNA increased in the hypophysectomized female rat liver, indicating that rOAT3 is an inducible isoform. The present findings suggest that sex steroids play an important role in the expression and maintenance of OAT2/3 isoforms in the rat liver and kidney. Our results provide information on the differential gene expression of OAT isoforms with sex hormone dependency.</description><subject>Animals</subject><subject>Blotting, Northern</subject><subject>Brain - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Kidney - metabolism</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>organic anion</subject><subject>Organic Anion Transport Protein 1 - biosynthesis</subject><subject>Organic Anion Transport Protein 1 - genetics</subject><subject>organic anion transporter</subject><subject>Organic Anion Transporters - biosynthesis</subject><subject>Organic Anion Transporters - genetics</subject><subject>Organic Anion Transporters, Sodium-Independent - biosynthesis</subject><subject>Organic Anion Transporters, Sodium-Independent - genetics</subject><subject>pharmacokinetics</subject><subject>Protein Isoforms</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>sex differences</subject><subject>Sex Factors</subject><subject>Tissue Distribution</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNp1kMFLwzAUh4Mobk6vHiUnb61JmqbNccxtCpPBmOAtpOmrRNq0Jp3of2_LBp48vcfj-_3gfQjdUhJTQsRDUXgTM0JoLGhOztCUEkkiRgk_R1MyEBGT9G2CrkL4GCjKhbxEE0qzTFIppmj3aKsKPLje6hqvwQFefnceQrCtw22Ft_5dO2vw3I2HvdcudK3vwQdsHX7RNWDtSryCZlx3ug_X6KLSdYCb05yh19Vyv3iKNtv182K-iQwnoo-krtLMyFzkWnLIy7RIpWAi0STNmS4KzRPGoCiSKtWSZTQTLAVemhI0VFCmyQzdH3s7334eIPSqscFAXWsH7SGojCZcMsYHMD6CxrcheKhU522j_Y-iRI0W1WhRjRbVaHEI3J2aD0UD5R9-0jYA-RGA4b8vC14FY8EZKK0H06uytf91_wJgxoEF</recordid><startdate>20020111</startdate><enddate>20020111</enddate><creator>Kobayashi, Yasuna</creator><creator>Hirokawa, Noriko</creator><creator>Ohshiro, Naomi</creator><creator>Sekine, Takashi</creator><creator>Sasaki, Tadanori</creator><creator>Tokuyama, Shogo</creator><creator>Endou, Hitoshi</creator><creator>Yamamoto, Toshinori</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20020111</creationdate><title>Differential Gene Expression of Organic Anion Transporters in Male and Female Rats</title><author>Kobayashi, Yasuna ; Hirokawa, Noriko ; Ohshiro, Naomi ; Sekine, Takashi ; Sasaki, Tadanori ; Tokuyama, Shogo ; Endou, Hitoshi ; Yamamoto, Toshinori</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-9af57c9868a94e8d5b596263a0582abba4322ebb3f5a92717625e4dcdeaefed53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Blotting, Northern</topic><topic>Brain - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Kidney - metabolism</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>organic anion</topic><topic>Organic Anion Transport Protein 1 - biosynthesis</topic><topic>Organic Anion Transport Protein 1 - genetics</topic><topic>organic anion transporter</topic><topic>Organic Anion Transporters - biosynthesis</topic><topic>Organic Anion Transporters - genetics</topic><topic>Organic Anion Transporters, Sodium-Independent - biosynthesis</topic><topic>Organic Anion Transporters, Sodium-Independent - genetics</topic><topic>pharmacokinetics</topic><topic>Protein Isoforms</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>sex differences</topic><topic>Sex Factors</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kobayashi, Yasuna</creatorcontrib><creatorcontrib>Hirokawa, Noriko</creatorcontrib><creatorcontrib>Ohshiro, Naomi</creatorcontrib><creatorcontrib>Sekine, Takashi</creatorcontrib><creatorcontrib>Sasaki, Tadanori</creatorcontrib><creatorcontrib>Tokuyama, Shogo</creatorcontrib><creatorcontrib>Endou, Hitoshi</creatorcontrib><creatorcontrib>Yamamoto, Toshinori</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kobayashi, Yasuna</au><au>Hirokawa, Noriko</au><au>Ohshiro, Naomi</au><au>Sekine, Takashi</au><au>Sasaki, Tadanori</au><au>Tokuyama, Shogo</au><au>Endou, Hitoshi</au><au>Yamamoto, Toshinori</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Gene Expression of Organic Anion Transporters in Male and Female Rats</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2002-01-11</date><risdate>2002</risdate><volume>290</volume><issue>1</issue><spage>482</spage><epage>487</epage><pages>482-487</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Sex-related differential gene expression of organic anion transporters (rOAT1, rOAT2, and rOAT3) in rat brain, liver, and kidney was investigated. There were no sex differences in the expression of rOAT1 mRNA. rOAT2 mRNA was abundant in the liver and weakly expressed in the kidney of male rats; however, the OAT2 gene was strongly expressed in both organs of females. The abundance of rOAT2 mRNA markedly increased in castrated male rat kidney; however, treatment of castrated male rats with testosterone led to a decrease of rOAT2 mRNA. Expression of rOAT3 mRNA in intact female rats was found in the kidney and brain, whereas in males rOAT3 mRNA was also found in the liver. rOAT3 mRNA markedly decreased in the liver of castrated male rats but increased in testosterone-treated castrated male rats. Moreover, rOAT3 mRNA increased in the hypophysectomized female rat liver, indicating that rOAT3 is an inducible isoform. The present findings suggest that sex steroids play an important role in the expression and maintenance of OAT2/3 isoforms in the rat liver and kidney. 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| source | ScienceDirect Journals |
| subjects | Animals Blotting, Northern Brain - metabolism Female Gene Expression Regulation Kidney - metabolism Liver - metabolism Male organic anion Organic Anion Transport Protein 1 - biosynthesis Organic Anion Transport Protein 1 - genetics organic anion transporter Organic Anion Transporters - biosynthesis Organic Anion Transporters - genetics Organic Anion Transporters, Sodium-Independent - biosynthesis Organic Anion Transporters, Sodium-Independent - genetics pharmacokinetics Protein Isoforms Rats Rats, Wistar RNA - metabolism RNA, Messenger - metabolism sex differences Sex Factors Tissue Distribution |
| title | Differential Gene Expression of Organic Anion Transporters in Male and Female Rats |
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