Cross-linked coatings for electrophoretic separations in poly(dimethylsiloxane) microchannels

We have developed a strategy using ultraviolet light to polymerize mixed monomer solutions onto the surface of a poly(dimethylsiloxane) (PDMS) microdevice. By including monomers with different chemical properties, electrophoretic separations were optimized for a test set of analytes. The properties...

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Bibliographic Details
Published in:Electrophoresis 2003-11, Vol.24 (21), p.3679-3688
Main Authors: Hu, Shuwen, Ren, Xueqin, Bachman, Mark, Sims, Christopher E., Li, G. P., Allbritton, Nancy
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Cross-linked polymer
Cross-Linking Reagents
Dimethylpolysiloxanes
Electrophoresis, Capillary - methods
Electrophoretic separation
Fluoresceins
Miniaturization
Miniaturization - methods
Molecular Probe Techniques
Molecular Sequence Data
Peptide Fragments - chemistry
Peptide Fragments - isolation & purification
Peptides - chemistry
Peptides - isolation & purification
Poly(dimethylsiloxane)
Poly(ethylene glycol)
Polyethylene Glycols
Silicones
Ultraviolet grafting
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Summary:We have developed a strategy using ultraviolet light to polymerize mixed monomer solutions onto the surface of a poly(dimethylsiloxane) (PDMS) microdevice. By including monomers with different chemical properties, electrophoretic separations were optimized for a test set of analytes. The properties of surfaces grafted with a single neutral monomer, a neutral and a negative monomer, or a neutral, negative, and cross‐linking monomer were assessed. The highest quality separations were achieved in channels with cross‐linked coatings. The separation efficiency for biologically relevant peptides (kinase substrates) on these surfaces was as high as 18 600 theoretical plates in a 2.5 cm channel. The test peptides were fluorescein‐AEEEIYGEFEAKKKK, fluorescein‐GRPRAATFAEG, fluorescein‐GRPRAA(T‐PO3)FAEG, fluorescein‐DLDVPIP GRFDRRVSVAAE, and fluorescein‐DLDVPIPGRFDRRV(S‐PO3)VAAE. Separations between two different peptides occurred in as little as 400 ms after injection into the separation channel. The simultaneous separation of five kinase and phosphatase substrates was also demonstrated. By carefully selecting mixtures of monomers with the appropriate properties, it may be possible to tailor the surface of PDMS for a large number of different electrophoretic separations.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200305592