Psoriasin expression in mammary epithelial cells in vitro and in vivo

• Published in: Cancer research (Chicago, Ill.) Ill.), 2002, Vol.62 (1), p.43-47
• Main Authors: ENERBÄCK, Charlotta, PORTER, Dale A, BARNHART, Kerry, POLYAK, Kornelia, SETH, Pankaj, SGROI, Dennis, GAUDET, Justine, WEREMOWICZ, Stanislawa, MORTON, Cynthia C, SCHNITT, Stuart, PITTS, Robert L, STAMPL, Jason
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Biological and medical sciences; Biomarkers, Tumor - biosynthesis; Biomarkers, Tumor - genetics; Biomarkers, Tumor - secretion; Breast - cytology; Breast - metabolism; Breast - physiology; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Calcium-Binding Proteins - biosynthesis; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - secretion; Carcinoma in Situ - genetics; Carcinoma in Situ - metabolism; Carcinoma, Ductal, Breast - genetics; Carcinoma, Ductal, Breast - metabolism; Cytoplasm - metabolism; Epithelial Cells - cytology; Epithelial Cells - metabolism; Female; Gene Expression Profiling; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry; Mammary gland diseases; Medical sciences; Molecular Sequence Data; Psoriasis - genetics; Psoriasis - metabolism; Psoriasis - pathology; Reverse Transcriptase Polymerase Chain Reaction; S100 Calcium Binding Protein A7; S100 Proteins; Subcellular Fractions - metabolism; Tumor Cells, Cultured; Tumors
• ISSN: 0008-5472; 1538-7445

We determined, by serial analysis of gene expression (SAGE) analysis of normal and DCIS (ductal carcinoma in situ) mammary epithelial cells, that psoriasin and several other genes implicated in psoriasis are aberrantly expressed in high-grade, comedo DCIS. Real-time PCR, mRNA in situ hybridization, and immunohistochemical analysis of breast carcinomas confirmed that psoriasin is frequently overexpressed in estrogen receptor-negative tumors. To gain insight into regulatory pathways that control psoriasin expression, we developed polyclonal and monoclonal antibodies and investigated mechanisms that may account for elevated levels of psoriasin in DCIS. Here, we report that loss of attachment to extracellular matrix, growth factor deprivation, and confluent conditions dramatically up-regulate psoriasin expression in MCF10A mammary epithelial cells. All of these conditions are characteristic of high-grade DCIS and psoriatic skin lesions; therefore, the same mechanisms may be responsible for increased expression of psoriasin in vitro and in vivo.