The tau protein of oral epithelium increases in Alzheimer's disease

Alzheimer's disease (AD) is an important problem that should be solved in the 21st century. Prior to treatment, a simple and easy diagnostic method using biological markers should be available. As a method to attain this goal, we detected and determined tau protein in oral mucosal epithelium. O...

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Bibliographic Details
Published in:The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2002-01, Vol.57 (1), p.M64-M70
Main Authors: Hattori, Hideyuki, Matsumoto, Masayuki, Iwai, Kunimitu, Tsuchiya, Hiroshi, Miyauchi, Eiji, Takasaki, Mikihiro, Kamino, Kouzin, Munehira, Junichi, Kimura, Yasuhiro, Kawanishi, Kenichi, Hoshino, Tomoko, Murai, Hiroshi, Ogata, Hajime, Maruyama, Hidemichi, Yoshida, Hiroshi
Format: Article
Language:English
Subjects:
Adult
Aged
Alzheimer Disease - metabolism
Blotting, Western
Dementia, Vascular - metabolism
Female
Humans
Male
Middle Aged
Molecular Weight
Mouth Mucosa - metabolism
Osmolar Concentration
Protein Isoforms - metabolism
Reference Values
tau Proteins - cerebrospinal fluid
tau Proteins - chemistry
tau Proteins - metabolism
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Summary:Alzheimer's disease (AD) is an important problem that should be solved in the 21st century. Prior to treatment, a simple and easy diagnostic method using biological markers should be available. As a method to attain this goal, we detected and determined tau protein in oral mucosal epithelium. Oral epithelium was exfoliated from 34 patients with AD or 29 patients with vascular dementia, and 33 young and 34 age-matched controls. Western blot was performed for determining the molecular weight of oral tau protein. The tau protein level was determined with an enzyme-linked immunosorbent assay (ELISA) kit for cerebrospinal fluid (CSF). CSF tau was also measured and compared with oral tau. Western blot analysis using an anti-non-phosphorylated tau-protein antibody showed two bands, one at 65 Kd and the other at 110 Kd. The tau-protein level in oral epithelia showed a significant positive correlation with those in the CSF (p
ISSN:1079-5006
DOI:10.1093/gerona/57.1.M64