Endogenous nandrolone metabolites in human urine: preliminary results to discriminate between endogenous and exogenous origin

When administered to human subjects, nandrolone is metabolized into two main products, 19-norandrosterone (19-NA) and 19-noretiocholanolone (19-NE). Recent studies demonstrated the endogenous production of these compounds in man at concentrations very close to the threshold of the International Olym...

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Bibliographic Details
Published in:Steroids 2002-02, Vol.67 (2), p.105-110
Main Authors: Le Bizec, Bruno, Bryand, Fabrice, Gaudin, Isabelle, Monteau, Fabrice, Poulain, Frédéric, Andre, François
Format: Article
Language:English
Subjects:
19-Norandrosterone
19-Noretiocholanolone
Adult
Anabolic
Biological and medical sciences
Chromatography, High Pressure Liquid
Conjugation
Diet
Doping
Endogenous
Estranes - metabolism
Gas Chromatography-Mass Spectrometry
Glucuronides - metabolism
Humans
Male
Middle Aged
Nandrolone
Nandrolone - administration & dosage
Nandrolone - metabolism
Nandrolone - urine
Substance Abuse Detection - methods
Sulfates - metabolism
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Summary:When administered to human subjects, nandrolone is metabolized into two main products, 19-norandrosterone (19-NA) and 19-noretiocholanolone (19-NE). Recent studies demonstrated the endogenous production of these compounds in man at concentrations very close to the threshold of the International Olympic Committee (IOC), i.e. 2 ng/ml. Because the possibility of reaching or exceeding this fateful limit is difficult to exclude, a complementary biochemical parameter is necessary for the differentiation of endogenous 19-NA and 19-NE production from residues resulting from nandrolone consumption. We measured the endogenous concentrations of 19-NA and 19-NE in 385 urine samples from professional football players, and we studied the phase II metabolite composition in individuals excreting the highest concentrations. The results showed that around 30% of endogenous 19-norandrosterone was sulfo-conjugated, whereas 100% of 19-norandrosterone was excreted conjugated to a glucuronic acid when nandrolone was administered. This significant qualitative difference appears to be a promising complementary criterion to more definitively conclude about an athlete’s culpability, especially when nandrolone metabolites are found in the low ng/ml range.
ISSN:0039-128X
1878-5867
DOI:10.1016/S0039-128X(01)00139-8