Alterations in the expression and modification of histones in the liver after injury

Chromatin remodeling plays a key role in the transcriptional activation of regulatory factors in the liver in response to a variety of stress signals. The effects of burn injury on histone expression and its modification were investigated in this study. Liver tissues collected after a flame burn inj...

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Bibliographic Details
Published in:Experimental and molecular pathology 2003-12, Vol.75 (3), p.256-264
Main Authors: JEONG, Jayoung, ADAMSON, Lee K, HATAM, Robin, GREENHALGH, David G, CHO, Kiho
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Western
Burns - physiopathology
Female
Histones - metabolism
Investigative techniques, diagnostic techniques (general aspects)
Liver - injuries
Liver - metabolism
Medical sciences
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Proliferating Cell Nuclear Antigen - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
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Summary:Chromatin remodeling plays a key role in the transcriptional activation of regulatory factors in the liver in response to a variety of stress signals. The effects of burn injury on histone expression and its modification were investigated in this study. Liver tissues collected after a flame burn injury were subjected to RT-PCR and Western blot analyses of histone regulation. There was a marked induction of histone H3-D variant mRNA at 3 and 6 h. In contrast, histone H2A.2 variant mRNA had a downregulation at 3 days. No apparent changes were noted in other histone variants examined. Western blot analysis revealed a downregulation of all 5 histone subtypes (H1, H2A, H2B, H3, and H4) at 1 day and there was a subsequent induction of H1 and H2A subtypes at 3 days after injury. There was an induction of modified forms (phospho-, acetyl-, and dimethyl-) of histone H3 subtype at day 3. Furthermore, a transient elevation in PCNA (proliferating cell nuclear antigen) levels was apparent in the liver at day 3, which parallels the induction of phospho-histone H3, which is a mitosis marker. These findings suggest that histones participate in a cascade of events associated with phenotypic alterations in the liver after injury.
ISSN:0014-4800
1096-0945
DOI:10.1016/S0014-4800(03)00095-9