Role of neuropeptide Y Y(2) receptors in modulation of cardiac parasympathetic neurotransmission

The aim of the study was to clarify the role of the Y(2) receptor in regulation of vagal control of the heart, using Y(2)((-/-)) receptor-knockout mice. Adult Y(2)((+/+),(-/-)) mice (50% C57BL/6-50% 129/SvJ background) were anaesthetised and artificially ventilated. Arterial blood pressure and pulse...

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Bibliographic Details
Published in:Regulatory peptides 2002-02, Vol.103 (2-3), p.105-111
Main Authors: Smith-White, Margaret A, Herzog, Herbert, Potter, Erica K
Format: Article
Language:English
Subjects:
Animals
Arginine - analogs & derivatives
Arginine - pharmacology
Benzazepines - pharmacology
Blood Pressure - drug effects
Dose-Response Relationship, Drug
Electrocardiography
Electrophysiology
Heart - drug effects
Heart - innervation
Mice
Mice, Knockout
Myocardium - metabolism
Neuropeptide Y - analogs & derivatives
Neuropeptide Y - pharmacology
Neurotransmitter Agents - metabolism
Parasympathetic Nervous System - drug effects
Parasympathetic Nervous System - metabolism
Parasympathetic Nervous System - physiology
Peptide Fragments - pharmacology
Receptors, Neuropeptide Y - agonists
Receptors, Neuropeptide Y - metabolism
Vagus Nerve - drug effects
Vagus Nerve - physiology
Vagus Nerve - surgery
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Summary:The aim of the study was to clarify the role of the Y(2) receptor in regulation of vagal control of the heart, using Y(2)((-/-)) receptor-knockout mice. Adult Y(2)((+/+),(-/-)) mice (50% C57BL/6-50% 129/SvJ background) were anaesthetised and artificially ventilated. Arterial blood pressure and pulse interval was recorded and both vagus nerves were cut. The cardiac end of the right vagus nerve was stimulated supra-maximally every 30 s (7 V, 2-2.5 Hz, 5 s). Neuropeptide Y (NPY) and a Y(2) receptor agonist, N-acetyl [Leu(28, 31)]NPY 24-36, were injected intravenously in both groups of mice. N-acetyl [Leu(28, 31)] NPY 24-36 was also administered to control mice in the presence of a Y(2) receptor antagonist, BIIE0246. Stimulation of the vagus nerve increased pulse interval (PI) by approximately 100 ms. NPY and N-acetyl [Leu(28, 31)] NPY 24-36 attenuated the increase in PI evoked by vagal stimulation in control mice only. The attenuation was reduced in the presence of BIIE0246. The results presented here show in Y(2)((-/-)) receptor-knockout mice that NPY and N-acetyl [Leu(28, 31)] NPY 24-36 have no effect on PI evoked by vagal stimulation. These findings demonstrate that NPY attenuates parasympathetic activity to the heart via the Y(2) receptor.
ISSN:0167-0115