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Expression of the genes for lysostaphin and lysostaphin resistance in streptococci

To determine if the genes for lysostaphin endopeptidase ( end) and lysostaphin resistance ( epr) function in streptococci, we transferred these genes from Staphylococcus simulans biovar staphylolyticus into two strains of Streptococcus equi subsp. zooepidemicus. The end-containing streptococci were...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-11, Vol.228 (1), p.115-119
Main Authors: Heath Farris, M, Heath, Lucie S, Heath, Harry E, LeBlanc, Paul A, Simmonds, Robin S, Sloan, Gary L
Format: Article
Language:English
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Summary:To determine if the genes for lysostaphin endopeptidase ( end) and lysostaphin resistance ( epr) function in streptococci, we transferred these genes from Staphylococcus simulans biovar staphylolyticus into two strains of Streptococcus equi subsp. zooepidemicus. The end-containing streptococci were able to produce and process proendopeptidase. Strains containing epr were more resistant to lysis by the streptococcolytic enzyme zoocin A and amino acid analysis of the peptidoglycans of the epr-containing streptococci revealed insertion of serines in their cross bridges. This is the first report of the transfer of a femABX-like immunity factor resulting in a physiologically useful effect in a different genus.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00743-2