PROCEDURE FOR SUCCESSFUL INTERSPECIFIC EMBRYO TRANSFER FROM MOUFLON (OVIS GMELINI MUSIMON) TO SPANISH MERINO SHEEP (OVIS ARIES)

Embryos from five anesthetized mouflons (Ovis gmelini musimon), superovulated with FSH-o (Ovagen™) were transferred into preselected Spanish Merino sheep (Ovis aries). Myorelaxation was complete in four of five donor mouflons. The status of the uterus of potential recipients was evaluated by transre...

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Bibliographic Details
Published in:Journal of zoo and wildlife medicine 2001-09, Vol.32 (3), p.336-341
Main Authors: Santiago-Moreno, J, González-Bulnes, A, Gómez-Brunet, A, Cocero, M. J, del Campo, A, García-García, R, López-Sebastián, A
Format: Article
Language:English
Subjects:
Anesthesia
Anesthetics, Dissociative
Animal reproduction
Animals
Animals, Domestic
Animals, Wild
Conservation of Natural Resources
Corpus luteum
Corpus Luteum - diagnostic imaging
Embryo transfer
Embryo Transfer - standards
Embryo Transfer - veterinary
Embryos
Estrus Synchronization - drug effects
Ewes
Female
Flurogestone Acetate - administration & dosage
Follicle Stimulating Hormone - administration & dosage
Hypnotics and Sedatives
Immobilization
Ketamine
Medetomidine
Mouflon
Ovis gmelini musimon
Pregnancy
Pregnancy Outcome - veterinary
progesterone
Progesterone - blood
Progesterone Congeners - administration & dosage
Sheep
Sheep - embryology
Sheep - physiology
Superovulation
Ultrasonography
Uterus - diagnostic imaging
Zoos
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Summary:Embryos from five anesthetized mouflons (Ovis gmelini musimon), superovulated with FSH-o (Ovagen™) were transferred into preselected Spanish Merino sheep (Ovis aries). Myorelaxation was complete in four of five donor mouflons. The status of the uterus of potential recipients was evaluated by transrectal ultrasonography, and those ewes with fluid in the uterine horn were rejected. The corpus luteum in each ewe was assessed ultrasonographically the day before surgery. Plasma progesterone levels and the quality of the corpora lutea were the criteria for selection of recipients. Ten embryos were transferred to the five selected Spanish Merino recipients, resulting in four pregnancies and seven live-born lambs, including three sets of twins. This study shows that determination of plasma progesterone levels combined with ultrasonographic assessment of the corpus luteum provides information useful for screening of potential recipients.
ISSN:1042-7260
1937-2825
DOI:10.1638/1042-7260(2001)032[0336:PFSIET]2.0.CO;2