Chondrogenic Potential of Adipose Tissue-Derived Stromal Cells in Vitro and in Vivo

Articular cartilage exhibits little intrinsic repair capacity, and new tissue engineering approaches are being developed to promote cartilage regeneration using cellular therapies. The goal of this study was to examine the chondrogenic potential of adipose tissue-derived stromal cells. Stromal cells...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2002-01, Vol.290 (2), p.763-769
Main Authors: Erickson, Geoffrey R., Gimble, Jeffrey M., Franklin, Dawn M., Rice, Henry E., Awad, Hani, Guilak, Farshid
Format: Article
Language:English
Subjects:
adipose tissue
Adipose Tissue - cytology
Animals
cartilage
Cartilage - cytology
Cartilage - metabolism
Cell Differentiation - physiology
Cells, Cultured
chondrocyte
Chondroitin Sulfates - biosynthesis
Collagen Type II - biosynthesis
Collagen Type VI - biosynthesis
Culture Techniques - methods
differentiation
Humans
Immunohistochemistry
mesenchymal stem cell
Mice
Mice, Nude
multipotent
pluripotent
Proteoglycans - biosynthesis
stem cell
stroma
Stromal Cells - cytology
Stromal Cells - metabolism
Stromal Cells - transplantation
tissue engineering
Transplantation, Heterologous
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Summary:Articular cartilage exhibits little intrinsic repair capacity, and new tissue engineering approaches are being developed to promote cartilage regeneration using cellular therapies. The goal of this study was to examine the chondrogenic potential of adipose tissue-derived stromal cells. Stromal cells were isolated from human subcutaneous adipose tissue obtained by liposuction and were expanded and grown in vitro with or without chondrogenic media in alginate culture. Adipose-derived stromal cells abundantly synthesized cartilage matrix molecules including collagen type II, VI, and chondroitin 4-sulfate. Alginate cell constructs grown in chondrogenic media for 2 weeks in vitro were then implanted subcutaneously in nude mice for 4 and 12 weeks. Immunohistochemical analysis of these samples showed significant production of cartilage matrix molecules. These findings document the ability of adipose tissue-derived stromal cells to produce characteristic cartilage matrix molecules in both in vitro and in vivo models, and suggest the potential of these cells in cartilage tissue engineering.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.6270