Lactobacilli express cell surface proteins which mediate binding of immobilized collagen and fibronectin

• Published in: FEMS microbiology letters 2002-01, Vol.206 (1), p.31-37
• Main Authors: Lorca, G, Torino, M.I, Font de Valdez, G, Ljungh, A
• Format: Article
• Language: English
• Subjects: adhesion; adverse effects; Amino acids; Animals; Bacterial Capsules - metabolism; Bacterial Proteins - metabolism; Bacteriology; Binding; Biological and medical sciences; Cell surface; Collagen; Collagen - metabolism; Collagen binding; Electrophoresis, Polyacrylamide Gel; Endopeptidase K; Extracellular matrix; Fibronectin; Fibronectin binding; fibronectins; Fibronectins - metabolism; Fundamental and applied biological sciences. Psychology; Gel electrophoresis; Gelatin; heat; Humans; Immunoblot; Immunoblotting; Lactobacilli; Lactobacillus; Lactobacillus acidophilus; Lactobacillus acidophilus - growth & development; Lactobacillus acidophilus - metabolism; Membrane Proteins - metabolism; Microbiology; Monoclonal antibodies; Morphology, structure, chemical composition; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; peptidase K; polyacrylamide gel electrophoresis; Polysaccharide; Pronase; Protein interaction; protein-protein interactions; Proteinase; Proteins; Protein–protein interaction; Sodium lauryl sulfate; Sugar; sugars; surface proteins; Trypsin; Western blotting
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2002.tb10982.x

Binding of immobilized collagen-I (Cn-I)and fibronectin (Fn) by Lactobacillus acidophilus CRL 639 depends on cell-surface proteins. Capsule formation during the stationary growth phase has a negative effect on adherence of Cn-I and Fn. However, cells from the exponential growth phase, which produce no capsule, exhibit maximal binding. Binding is sensitive to trypsin, proteinase K, pronase E, and heat. Gelatin and soluble Cn-I partially inhibit binding of Cn-I although various proteins, sugars and amino acids do not affect binding to Fn. These results indicate that protein-protein interactions mediate adhesion to extracellular matrix proteins. SDS-PAGE and Western blot analyses of surface proteins revealed that several proteins including the major 43-kDa protein of the S-layer are expressed. Monoclonal antibodies showed that Fn binds to a 15-kDa protein, while Cn-I binds to proteins of 45 and 58 kDa.