Comparative study on the susceptibility of cutworms (Lepidoptera: Noctuidae) to Agrotis segetum nucleopolyhedrovirus and Agrotis ipsilon nucleopolyhedrovirus

The common cutworm ( Agrotis segetum) and the black cutworm ( Agrotis ipsilon) are serious soil pests of many vegetable and field crops all over the world. We have demonstrated the cross-infectivity of two baculoviruses, A. segetum nucleopolyhedrovirus (AgseNPV) and A. ipsilon nucleopolyhedrovirus (...

Full description

Saved in:
Bibliographic Details
Published in:Journal of invertebrate pathology 2003-10, Vol.84 (2), p.75-82
Main Authors: El-Salamouny, Said, Lange, Martin, Jutzi, Manfred, Huber, Jürg, Jehle, Johannes A.
Format: Article
Language:English
Subjects:
Agrotis ipsilon
Agrotis ipsilon nucleopolyhedrovirus
Agrotis segetum
Agrotis segetum nucleopolyhedrovirus
Animals
Base Sequence
Biological and medical sciences
Biological control
Control
Cross-transmission
DNA Restriction Enzymes
DNA, Viral - analysis
Fundamental and applied biological sciences. Psychology
Lepidoptera - virology
Microbiology
Molecular Sequence Data
Noctuidae
Nuclear polyhedrosis virus
Nucleopolyhedrovirus - genetics
Nucleopolyhedrovirus - pathogenicity
Pest Control, Biological
Phylogeny
Phytopathology. Animal pests. Plant and forest protection
polyhedrin
Polyhedrin sequence
Polymerase Chain Reaction
Protozoa. Invertebrates
Sequence Analysis, DNA
Spodoptera exigua
Spodoptera frugiperda
Spodoptera littoralis
Systematics
Viral Proteins - genetics
Viral Structural Proteins
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The common cutworm ( Agrotis segetum) and the black cutworm ( Agrotis ipsilon) are serious soil pests of many vegetable and field crops all over the world. We have demonstrated the cross-infectivity of two baculoviruses, A. segetum nucleopolyhedrovirus (AgseNPV) and A. ipsilon nucleopolyhedrovirus (AgipNPV) for these two insect pests. The susceptibility of A. segetum to AgipNPV was confirmed by DNA restriction endonuclease analyses of DNA isolated from virus harvested from infected A. segetum larvae. For an initial comparison of both viruses, partial polyhedrin sequences were amplified by PCR, cloned, and sequenced. Both viruses shared a very similar polyhedrin gene sequence resulting in only three amino acid substitutions. Phylogenetic analyses clearly demonstrated that both viruses belong to NPV group II and are most closely related to a clade consisting of Spodoptera exigua NPV, Spodoptera frugiperda NPV, and Spodoptera littoralis NPV. Since AgipNPV shows high virulence for both cutworm species, it appears to be a suitable candidate as a single biological control agent of A. segetum and A. ipsilon.
ISSN:0022-2011
1096-0805
DOI:10.1016/j.jip.2003.08.005