A Calcium-Dependent Protein Kinase Is Systemically Induced upon Wounding in Tomato Plants

• Published in: Plant physiology (Bethesda) 2002-01, Vol.128 (1), p.256-270
• Main Authors: CHICO, José Manuel, RAICES, Marcela, TELLEZ-INON, Maria Teresa, ULLOA, Rita Maria
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological; Amino Acid Sequence; Amino acids; Animal and plant ecology; Animal, plant and microbial ecology; Autoecology; Biological and medical sciences; Blotting, Northern; Blotting, Southern; Blotting, Western; Calcium; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - isolation & purification; Calcium-Binding Proteins - metabolism; Cell Biology and Signal Transduction; Cloning, Molecular; Colletotrichum - growth & development; DNA; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic; Generalities. Disease free stocks; Hydrogen peroxide; Immunity, Innate - genetics; Leaves; Lycopersicon esculentum - enzymology; Lycopersicon esculentum - genetics; Messenger RNA; Molecular Sequence Data; Particulate matter; Pathogens; Phytopathology. Animal pests. Plant and forest protection; Plant cells; Plant Diseases - microbiology; Plant Leaves - enzymology; Plant Leaves - genetics; Plants; Plants and fungi; Protein Kinases - genetics; Protein Kinases - isolation & purification; Protein Kinases - metabolism; Proteins; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Signal Transduction; Tomatoes
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.010649

A full-length cDNA clone (LeCDPK1) from tomato (Lycopersicon esculentum) encoding a calcium-dependent protein kinase (CDPK) was isolated by screening a cDNA library from tomato cell cultures exposed to Cladosporium fulvum elicitor preparations. The predicted amino acid sequence of the cDNA reveals a high degree of similarity with other members of the CDPK family. LeCDPK1 has a putative N-terminal myristoylation sequence and presents a possible palmitoylation site. The in vitro translated protein conserves the biochemical properties of a member of the CDPK family. In addition, CDPK activity was detected in soluble and particulate extracts of tomato leaves. Basal levels of LeCDPK1 mRNA were detected by northern-blot analysis in roots, stems, leaves, and flowers of tomato plants. The expression of LeCDPK1 was rapidly and transiently enhanced in detached tomato leaves treated with pathogen elicitors and H2O2. Moreover, when tomato greenhouse plants were subjected to mechanical wounding, a transient increase of LeCDPK1 steady-state mRNA levels was detected locally at the site of the injury and systemically in distant non-wounded leaves. The increase observed in LeCDPK1 mRNA upon wounding correlates with an increase in the amount and in the activity of a soluble CDPK detected in extracts of tomato leaves, suggesting that this kinase is part of physiological plant defense mechanisms against biotic or abiotic attacks.