Inactivation of the Mycobacterium bovis homologue of the polymorphic RD1 gene Rv3879c (Mb3909c) does not affect virulence

The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role...

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Bibliographic Details
Published in:Tuberculosis (Edinburgh, Scotland) Scotland), 2003, Vol.83 (6), p.387-393
Main Authors: Inwald, Jacqueline, Jahans, Keith, Hewinson, R.Glyn, Gordon, Stephen V
Format: Article
Language:English
Subjects:
Animals
Base Sequence
BCG Vaccine - immunology
Comparative genomics
Female
Gene Silencing - physiology
Genes, Bacterial - genetics
Guinea Pigs
Mutation - genetics
Mycobacterium bovis
Mycobacterium bovis - genetics
Mycobacterium bovis - pathogenicity
Phenotype
Polymorphism, Genetic - genetics
RD1 gene
Rv3879c gene
Tuberculosis - microbiology
Virulence
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Summary:The RD1 locus is deleted from all strains of Mycobacterium bovis BCG but present in virulent isolates of M. bovis and Mycobacterium tuberculosis. The RD1 gene Rv3879c encodes a proline- and alanine-rich protein that shows sequence polymorphism across members of the M. tuberculosis complex. The role of this protein in virulence was investigated by deleting the Rv3879c homologue from M. bovis (Mb3909c) and testing the virulence of the mutant in the guinea pig model. The M. bovis ΔMb3909c mutant was not attenuated in the guinea pig model, showing that this gene does not encode a virulence factor and plays no role in the attenuation caused by loss of RD1.
ISSN:1472-9792
1873-281X
DOI:10.1016/j.tube.2003.08.018