Reinduction of T-type calcium channels by endothelin-1 in failing hearts in vivo and in adult rat ventricular myocytes in vitro

In ventricular myocardium, the T-type Ca2+ current (ICa,T), which is temporarily observed during fetal and neonatal periods, has been shown to reappear in failing/remodeling hearts. However, its pathophysiological regulation has not been elucidated. We utilized Dahl salt-sensitive (DS) rats with hyp...

Full description

Saved in:
Bibliographic Details
Published in:Circulation (New York, N.Y.) N.Y.), 2003-11, Vol.108 (20), p.2530-2535
Main Authors: IZUMI, Toshiaki, KIHARA, Yasuki, SARAI, Nobuaki, YONEDA, Takeshi, IWANAGA, Yoshitaka, INAGAKI, Koichi, ONOZAWA, Yoko, TAKENAKA, Hiroyuki, KITA, Toru, NOMA, Akinori
Format: Article
Language:English
Subjects:
Angiotensin II - pharmacology
Animals
Antihypertensive Agents - pharmacology
Biological and medical sciences
Calcium Channels, T-Type - drug effects
Calcium Channels, T-Type - metabolism
Cardiology. Vascular system
Cells, Cultured
Disease Models, Animal
Endothelin Receptor Antagonists
Endothelin-1 - pharmacology
Heart
Heart Failure - drug therapy
Heart Failure - metabolism
Heart failure, cardiogenic pulmonary edema, cardiac enlargement
Heart Ventricles - cytology
Heart Ventricles - metabolism
Male
Medical sciences
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - metabolism
Rats
Rats, Inbred Dahl
Rats, Sprague-Dawley
Sulfonamides - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In ventricular myocardium, the T-type Ca2+ current (ICa,T), which is temporarily observed during fetal and neonatal periods, has been shown to reappear in failing/remodeling hearts. However, its pathophysiological regulation has not been elucidated. We utilized Dahl salt-sensitive (DS) rats with hypertension at the stage of concentric left ventricular (LV) hypertrophy (11 weeks old, LVH) and at the heart failure stage (16 to 18 weeks old, CHF). Some were treated with bosentan (100 mg/kg per day) during the period from LVH to CHF. In LVH, neither the presence of ICa,T (measured in the freshly isolated LV myocytes) nor an increase in alpha-1G mRNA expression were detected. This condition was associated with increases in tissue angiotensin II (AII) but not with endothelin (ET)-1 peptides. In contrast, in CHF, when the tissue AII remained elevated and ET-1 de novo increased, ICa,T was recorded in most of the cells (-0.87+/-0.18 pA/pF at -30 mV, P
ISSN:0009-7322
1524-4539
DOI:10.1161/01.CIR.0000096484.03318.AB