Variations in the ability of adsorbed fibrinogen to mediate platelet adhesion to polystyrene-based materials: A multivariate statistical analysis of antibody binding to the platelet binding sites of fibrinogen

• Published in: Journal of biomedical materials research 2003-12, Vol.67A (4), p.1255-1268
• Main Authors: Tsai, Wei-Bor, Grunkemeier, John M., Horbett, Thomas A.
• Format: Article
• Language: English
• Subjects: Adsorption; Animals; Antibodies, Monoclonal - metabolism; Binding Sites; Biological and medical sciences; biomaterials; Blood Platelets - metabolism; dodecapeptides; fibrinogen; Fibrinogen - chemistry; Fibrinogen - metabolism; fibrinogen adsorption; Humans; Medical sciences; Models, Biological; monoclonal antibody binding; Multivariate Analysis; Peptides - metabolism; Plasma - metabolism; platelet adhesion; Platelet Adhesiveness - physiology; platelet binding sites; Polystyrenes - chemistry; Polystyrenes - metabolism; Protein Binding; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Statistics as Topic; Surface Properties; Technology. Biomaterials. Equipments. Material. Instrumentation
• ISSN: 1549-3296; 0021-9304; 1552-4965; 1097-4636
• DOI: 10.1002/jbm.a.20024

Platelet adhesion to the surfaces of biomaterials preadsorbed with plasma previously has been shown to be mediated exclusively by surface‐bound fibrinogen and does not seem to involve the other adhesion proteins in plasma (Tsai et al., J Biomed Mater Res 2002;60:348–359). In this study, the influence of surface‐bound fibrinogen on platelet adhesion to five different types of polystyrene‐based microtiter plates preadsorbed with plasma was analyzed relative to the amount of adsorbed fibrinogen and monoclonal antibody binding to the adsorbed fibrinogen. There was no significant correlation between platelet adhesion and the absolute amount of adsorbed fibrinogen. However, platelet adhesion was positively correlated to the ability of the adsorbed fibrinogen to bind three types of monoclonal antibodies. The antibodies used bound to the sites on fibrinogen thought to be involved in platelet binding (the two γ chain C‐terminal dodecapeptides and the RGDF and RGDS sequences in each of the Aα chains). A partial least‐squares calibration model was used to analyze the relative importance of these binding sites in fibrinogen to platelet adhesion. The γ chain C‐terminal dodecapeptide was shown to be the most important site in adsorbed fibrinogen in mediating platelet adhesion. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1255–1268, 2003