Characterization and quantification of chitosan extracted from nacre of the abalone Haliotis tuberculata and the oyster Pinctada maxima

This study was performed to characterize and quantify chitosan by simple physicochemical methods (infrared spectroscopy and potentiometric measurements). These procedures were validated with well-characterized chitosan before being used to investigate chitosan in nacre of the abalone Haliotis tuberc...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2001-01, Vol.3 (1), p.36-44
Main Authors: Zentz, F, Bédouet, L, Almeida, M J, Milet, C, Lopez, E, Giraud, M
Format: Article
Language:English
Subjects:
Analytical methods
Calcium carbonate
Chitin
Chitosan
Deacetylation
Haliotis tuberculata
Infrared spectroscopy
Marine
Marine molluscs
Nacre
Oysters
Pearls
Pinctada maxima
Procedures
Proteins
Yields
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Summary:This study was performed to characterize and quantify chitosan by simple physicochemical methods (infrared spectroscopy and potentiometric measurements). These procedures were validated with well-characterized chitosan before being used to investigate chitosan in nacre of the abalone Haliotis tuberculata and of the giant oyster Pinctada maxima. Potentiometric study revealed a chitosan extract from the nacre of H. tuberculata with a degree of deacetylation of around 88% and an intrinsic pK of 6.5. According to infrared and potentiometric data, a low yield (eta) of extraction was calculated (eta = 0.064%). For experiments performed on the nacre of P. maxima, and in spite of more stringent deacetylation conditions, results suggested that a chitin-protein complex (eta = 0.053%) was isolated rather than chitosan.
ISSN:1436-2228
1436-2236
DOI:10.1007/s101260000042